Effects of energy expenditure and Ucp1 on photoperiod-induced weight gain in collared lemmings
Effects of energy expenditure and Ucp1 on photoperiod-induced weight gain in collared lemmings
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DOI：
10.1038/oby.2002.73
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发表时间：
2002-06-01
期刊：
影响因子：
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通讯作者：
Nagy, TR
中科院分区：
文献类型：
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作者：
Powell, CS;Blaylock, ML;Nagy, TR
目的：探讨总能量消耗（TEE）及其组分在带圈旅鼠因光周期减少而增加体重中的作用。研究方法和程序：采用24小时间接量热法测量能量消耗，同时进行食物摄入研究。通过协方差分析（ANCOVA）对TEE、静息和非静息能量消耗（REE和NREE）进行体重调整。采用Northern blot法检测肩胛间棕色脂肪组织解偶联蛋白1 (Ucp1) mRNA水平。结果：短光期10 d旅鼠TEE和REE显著低于长光期旅鼠（p < 0.05），而NREE无显著差异（p = 0.44）。短光周期旅鼠肩胛间棕色脂肪组织中Ucp1 mRNA水平比长光周期旅鼠低50% （p < 0.01）。Ucp1 mRNA水平与REE呈正相关（r(2) = 0.79, p < 0.01）。经REE校正Ucp1 mRNA水平差异后，光周期处理导致的差异不再显著（p = 0.54）。讨论：本研究的结果表明，当领鼠暴露于短光周期时，体重的增加可能主要是由REE的减少引起的，并与Ucp1 mRNA水平的降低相关。
Objective: To determine the role of total energy expenditure (TEE) and its components in the ability of collared lemmings to increase weight in response to a decrease in photoperiod.Research Methods and Procedures: Energy expenditure was measured by 24-hour indirect calorimetry concurrent with food-intake studies. TEE and resting and nonresting energy expenditure (REE and NREE, respectively) were adjusted for body weight by analysis of covariance (ANCOVA). Uncoupling protein 1 (Ucp1) mRNA levels from interscapular brown adipose tissue were determined by Northern blot.Results: TEE and REE of lemmings exposed to a short photoperiod for 10 days were significantly lower than that of lemmings exposed to a long photoperiod (p < 0.05), whereas NREE was not significantly different (p = 0.44). Ucp1 mRNA levels in interscapular brown adipose tissue were 50% lower in short- vs. long-photoperiod lemmings (p < 0.01). Ucp1 mRNA levels were positively related to REE (r(2) = 0.79, p < 0.01). After adjustment of REE for differences in Ucp1 mRNA levels, there was no longer a significant difference attributable to photoperiod treatment (p = 0.54).Discussion: The results of this study indicate that the increase in body mass that occurs when collared lemmings are exposed to a short photoperiod may be primarily fueled by a decrease in REE and is correlated with a decrease in Ucp1 mRNA levels.