Orally administered decoction of Kampo (Japanese herbal) medicine, "Juzen-Taiho-To" modulates cytokine secretion and induces NKT cells in mouse liver
Orally administered decoction of Kampo (Japanese herbal) medicine, "Juzen-Taiho-To" modulates cytokine secretion and induces NKT cells in mouse liver
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DOI：
10.1016/s0162-3109(99)00166-6
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发表时间：
2000-02-01
期刊：
影响因子：
--
通讯作者：
Yamada, H
中科院分区：
文献类型：
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作者：
Matsumoto, T;Sakurai, MH;Yamada, H
在小鼠中研究了口服十全大宝汤汤剂对肝淋巴细胞细胞因子产生的影响。研究发现，JTT 可以增加受刺激肝淋巴细胞的干扰素 γ (IFN-gamma) 以及白细胞介素 4 (IL-4)、IL-5 和 IL-6 的分泌，而 IL-2 的分泌则减少。 JTT 的施用并未改变 IFN-γ 和 IL-4 斑点形成细胞 (SFC) 的数量。这些结果表明，JTT对细胞因子分泌的调节可能不是由于肝淋巴细胞内细胞因子分泌细胞数量的变化所致，肝淋巴细胞中CD4/CD8比率和αβ/γδT细胞受体(TCR)比率没有改变。然而，流式细胞术分析显示，口服JTT后，NK阳性细胞（NKT细胞）中CD3阳性中间细胞的数量增加。 CD3(int)IL-2R beta(+) 细胞的数量也有所增加。注射 2-氯腺苷可减少 JTT 对 NKT 细胞的诱导作用。 JTT 增强肝脏中 IL-12 mRNA 的转录。从这些结果可以得出结论，NKT 细胞群的增加至少部分地促进了 JTT 对肝淋巴细胞和巨噬细胞中细胞因子产生的调节作用。肝脏中 IL-12 的产生也可能有助于这种 NKT 诱导。 (C) 2000 Elsevier Science B.V. 保留所有权利。
The effects of orally administered decoction of Juzen-Taiho-To (JTT; Si-Quan-Da-Bu-Tang in Chinese) on cytokine production in hepatic lymphocytes were studied in mice. JTT was found to increase interferon gamma (IFN-gamma), as well as interleukin-4 (IL-4), IL-5 and IL-6 secretion from stimulated hepatic lymphocytes, whereas IL-2 secretion was reduced. The number of IFN-gamma- and IL-4-spot forming cells (SFC) were not changed by administration of JTT. These results suggest that modulation of cytokine secretion by JTT might not be due to changes in the number of cytokine secreting cells within liver lymphocytes, CD4/CD8 ratio and alpha beta/gamma delta T cell, receptor (TCR) ratio in hepatic lymphocytes were not changed. However, flow cytometric analysis revealed that the population of CD3 positive intermediate cells in NK positive cells (NKT cells) was increased after oral administration of JTT. The population of CD3(int)IL-2R beta(+) cells was also increased. The induction of NKT cells by JTT was reduced by injection of 2-chloroadenosine. JTT enhanced transcription of IL-12 mRNA in Liver. From these results, it may be concluded that a rise in NKT cell population contributes, at least partially, to the modulating effect of JTT on cytokine production in liver lymphocytes, and macrophages. The production of IL-12 in liver may also contribute to this NKT induction. (C) 2000 Elsevier Science B.V. All rights reserved.