Neuropeptide discovery in Eucyclops serrulatus (Crustacea, Copepoda): In Silico prediction of the first peptidome for a member of the Cyclopoida
Neuropeptide discovery in Eucyclops serrulatus (Crustacea, Copepoda): In Silico prediction of the first peptidome for a member of the Cyclopoida
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DOI：
10.1016/j.ygcen.2014.11.002
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发表时间：
2015-01-15
影响因子：
2.7
通讯作者：
Christie, Andrew E.
中科院分区：
文献类型：
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作者：
Christie, Andrew E.
桡足亚纲的甲壳类动物基本上是所有水生生态系统的关键组成部分，因为它们既是浮游植物的主要消费者，又是各种高级消费者的主要食物来源。大多数淡水生态系统中桡足类的主要类群是桡足类。该目的成员通常被用作环境指标，一些捕食性物种被用于对致病蚊子进行生物控制。鉴于其生态和疾病控制的重要性，令人惊讶的是人们对环类内分泌控制知之甚少。在这里，作为识别和表征桡足类成员的神经化学信号系统的持续努力的一部分，对巨猿纲成员锯齿真目的现有转录组鸟枪组件进行了挖掘，以寻找编码假定的肽激素编码转录本的转录本。通过使用已知的节肢动物前/前激素原序列（主要来自其他桡足类物种）进行查询，鉴定出 36 个 E. serrulatus 肽编码转录本。从这些序列推导的蛋白质可以预测160种独特的成熟神经肽，包括色素分散激素的第一个桡足类同工型，以及脂肪运动激素-corazonin样肽的同工型、尿抑素A、尿抑素B、尿抑素C、尿泌素、甲壳类高血糖激素、利尿激素31、DXXRLamide、FLRFamide、 FXGGXamide、GSEFLamide、胰岛素样肽、intocin、白细胞激肽、肌抑制素、神经解蛋白、神经肽 F 和速激肽相关肽。这些肽是目前唯一已知的来自巨线虫的任何成员的肽，因此，为研究这一重要桡足类目中的肽能信号传导提供了新的资源。 (C) 2014 Elsevier Inc. 保留所有权利。
Crustaceans of the subclass Copepoda are key components of essentially all aquatic ecosystems as they serve both as the primary consumers of phytoplankton and/or as major food sources for a wide variety of higher-level consumers. The dominant group of copepods in most freshwater ecosystems is the Cyclopoida; members of this order are routinely used as environmental indicators, and some predatory species are used for the biological control of disease-causing mosquitoes. Given their ecological and disease control importance, it is surprising that little is known about endocrine control in cyclopoids. Here, as part of an ongoing effort to identify and characterize the neurochemical signaling systems of members of the Copepoda, the extant transcriptome shotgun assembly for Eucyclops serrulatus, a member of the Cyclopoida, was mined for transcripts encoding putative peptide hormone-encoding transcripts. Via queries using known arthropod pre/preprohormone sequences, primarily ones from other copepod species, 36 E. serrulatus peptide-encoding transcripts were identified. The proteins deduced from these sequences allowed for the prediction of 160 unique mature neuropeptides, including the first copepod isoform of pigment dispersing hormone, as well as isoforms of adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, allatotropin, crustacean hyperglycemic hormone, diuretic hormone 31, DXXRLamide, FLRFamide, FXGGXamide, GSEFLamide, insulin-like peptide, intocin, leucokinin, myosuppressin, neuroparsin, neuropeptide F and tachykinin-related peptide. These peptides are currently the only ones known from any member of the Cyclopoida, and as such, provide a new resource for investigating peptidergic signaling in this important copepod order. (C) 2014 Elsevier Inc. All rights reserved.