Asymptomatic seminal infection of herpes simplex virus: impact on male infertility.
Asymptomatic seminal infection of herpes simplex virus: impact on male infertility.
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单纯疱疹病毒的无症状精液感染：对男性不育症的影响。
DOI：
10.7555/jbr.27.20110139
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发表时间：
2013-01
影响因子：
2.3
通讯作者：
Fazlalipour M
中科院分区：
文献类型：
--
作者：
Monavari SH;Vaziri MS;Khalili M;Shamsi-Shahrabadi M;Keyvani H;Mollaei H;Fazlalipour M
在超过一半的不育男性中，他们不育的原因不明。几项研究揭示了病毒感染在男性不育症中的作用。本研究的目的是确定无症状不育男性患者精液中单纯疱疹病毒1型（HSV-1）和HSV-2的流行率，及其与精液参数改变的关系。从参加伊朗亚兹德不孕症研究和临床中心的不孕症男性中收集了总共70份精液样本。使用HSV-1和HSV-2 DNA的特异性引物和探针进行精液分析和诊断性实时PCR。用独立t检验和Mann-Whitney检验比较病毒感染与非病毒感染精液参数。精液分析结果表明，男性不育症可分为男性因素组和原因不明组。在70份精液标本中，HSV-1和HSV-2 DNA的检出率分别为22.9%（16/70）和14.3%（10/70）。所有HSV阳性的样本都有异常的精液参数（男性因素组）。虽然HSV感染与精子活力和形态缺陷无关，但与精液中精子计数降低相关。这些发现表明，HSV的无症状精液感染通过不利地影响精子计数在男性不育中起着重要作用。
In more than half of infertile men, the cause of their infertility is unknown. Several studies revealed the role of viral infections in male infertility. The aim of the present study was to determine the prevalence of herpes simplex virus-1 (HSV-1) and HSV-2 in semen from asymptomatic infertile male patients, and its association with altered semen parameters. A total of 70 semen samples were collected from infertile men who attended the Research and Clinical Center for Infertility in Yazd, Iran. Semen analysis and diagnostic real-time PCR using specific primers and probes for HSV-1 and HSV-2 DNA were performed. Comparison of semen parameters between virally infected and non-infected samples were performed with independent t-test and Mann-Whitney test. Semen analysis showed that infertile men fell into two groups, the male factor group and the unexplained group. HSV-1 and HSV-2 DNA was detected in 16 (22.9%) and 10 (14.3%) of 70 semen samples, respectively. All HSV-positive samples had abnormal semen parameters (the male factor group). Although HSV infection was not associated with sperm motility and morphological defects, it was correlated with lower sperm count in the seminal fluid. The findings suggest that asymptomatic seminal infection of HSV plays an important role in male infertility by adversely affecting sperm count.