Initiation of translation at internal AUG codons in mammalian cells
Initiation of translation at internal AUG codons in mammalian cells
复制标题
哺乳动物细胞中内部 AUG 密码子的翻译起始
作者：
Chung‐Cheng Liu;C. Simonsen;A. Levinson
真核 mRNA 的翻译起始通常发生在信息 5' 端的第一个 AUG 三联体处，尽管已经描述了几个值得注意的例外1-3。使用在猴细胞中有效表达编码乙型肝炎病毒表面抗原的基因的载体，我们研究了在该基因的通常翻译起始密码子上游的所有三个读码框中插入ATG三联体的后果。与真核翻译起始的扫描模型一致1,2，这些额外的密码子可以严重抑制“真实”起始密码子的翻译起始，尽管抑制的程度取决于上游 AUG 侧翼的序列。然而，这种抑制至少可以部分地被上游 AUG 符合读框的翻译终止密码子的存在所抑制。这些结果提出了一种可能性，即哺乳动物核糖体可以在先前在上游位点起始和终止后在AUG密码子处重新起始翻译。
Initiation of translation of eukaryotic mRNAs typically occurs at the first AUG triplet from the 5′ end of the message, although several notable exceptions have been described1–3. Using vectors which efficiently express the gene encoding the surface antigen of hepatitis B virus in monkey cells, we have studied the consequences of inserting ATG triplets in all three reading frames upstream of the usual translational initiation codon of this gene. In agreement with the scanning model for eukaryotic translation initiation1,2, these additional codons can severely depress the initiation of translation at the ‘authentic’ start codon, although the extent of inhibition depends on sequences flanking the upstream AUG. Such inhibition can, however, be at least partially suppressed by the presence of a translation termination codon in-frame with the upstream AUG. These results raise the possibility that mammalian ribosomes can reinitiate translation at an AUG codon after previously initiating, and terminating, at an upstream site.
控制哺乳动物细胞中细菌基因表达的因素。
影响因子：
5.3
作者：
Mulligan,RC;Berg,P
通讯作者：
Berg,P