Differences in Maternal Immunoglobulins within Mother's Own Breast Milk and Donor Breast Milk and across Digestion in Preterm Infants
Differences in Maternal Immunoglobulins within Mother's Own Breast Milk and Donor Breast Milk and across Digestion in Preterm Infants
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DOI：
10.3390/nu11040920
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发表时间：
2019-04-01
期刊：
影响因子：
5.9
通讯作者：
Dallas, David C.
中科院分区：
文献类型：
--
作者：
Demers-Mathieu, Veronique;Huston, Robert K.;Dallas, David C.
母体抗体转移给新生儿为婴儿的初始免疫系统提供了必要的支持。早产儿通常通过母亲自己的母乳 (MBM) 获得母体抗体，或者当母亲无法提供所需的所有乳汁时，通过捐赠母乳 (DBM) 获得母体抗体。 DBM 经过巴氏灭菌并经历多次冻融循环，这可能会降低婴儿体内完整抗体的浓度和抗体对消化的抵抗力。 MBM 和 DBM 中的抗体浓度是否不同以及它们在早产儿消化过程中的存活率是否不同仍然未知。饲料（MBM 或 DBM）、胃内容物（摄入后 1 小时的 MBM 或 DBM）和粪便样本（MBM 和 DBM 混合喂养后收集）是从产后 8-9 天和 21-22 天的 20 对早产儿（胎龄 26-36 周）母婴中收集的。通过 ELISA 分析样品中分泌型 IgA (SIgA)、总 IgA (SIgA/IgA)、总 IgM (SIgM/IgM) 和 IgG 的浓度。 MBM中的总IgA、SIgA、总IgM和IgG浓度比DBM中的总IgA、SIgA、总IgM和IgG浓度分别高出55.0%、71.6%、98.4%和41.1%，并且饲喂MBM的婴儿比饲喂DBM的婴儿的胃内容物分别高49.8%、32.7%、73.9%和39.7%。母乳中存在的所有母体抗体同种型均在婴儿粪便中检测到，其中 IgA（而非 sIgA）含量最丰富。
Maternal antibody transfer to the newborn provides essential support for the infant's naive immune system. Preterm infants normally receive maternal antibodies through mother's own breast milk (MBM) or, when mothers are unable to provide all the milk required, donor breast milk (DBM). DBM is pasteurized and exposed to several freeze-thaw cycles, which could reduce intact antibody concentration and the antibody's resistance to digestion within the infant. Whether concentrations of antibodies in MBM and DBM differ and whether their survival across digestion in preterm infants differs remains unknown. Feed (MBM or DBM), gastric contents (MBM or DBM at 1-h post-ingestion) and stool samples (collected after a mix of MBM and DBM feeding) were collected from 20 preterm (26-36 weeks gestational age) mother-infant pairs at 8-9 and 21-22 days of postnatal age. Samples were analyzed via ELISA for the concentration of secretory IgA (SIgA), total IgA (SIgA/IgA), total IgM (SIgM/IgM) and IgG. Total IgA, SIgA, total IgM and IgG concentrations were 55.0%, 71.6%, 98.4% and 41.1% higher in MBM than in DBM, and were 49.8%, 32.7%, 73.9% and 39.7% higher in gastric contents when infants were fed with MBM than when infants were fed DBM, respectively. All maternal antibody isotypes present in breast milk were detected in the infant stools, of which IgA (not sIgA) was the most abundant.