Production and biochemical characterization of an α-amylase from the moderate halophile Halomonas meridiana
Production and biochemical characterization of an α-amylase from the moderate halophile Halomonas meridiana
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DOI：
10.1111/j.1574-6968.2000.tb08935.x
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发表时间：
2000-02-01
影响因子：
2.1
通讯作者：
Nieto, JJ
中科院分区：
文献类型：
--
作者：
Coronado, MJ;Vargas, C;Nieto, JJ
优化了中度嗜盐菌Halomonasdamana产胞外淀粉酶的条件，并对该酶进行了生化表征。最高的淀粉酶产量是通过生长H。在不存在葡萄糖的情况下，在含有5%盐和淀粉的培养基中培养Escherichana，直到指数期结束。该淀粉酶在pH 7.0时活性最高，在碱性条件下相对稳定。最适温度和盐度分别为37 ℃和10%NaCl。此外，在盐度高达30%的盐的活性被检测到。麦芽糖和麦芽三糖是淀粉水解的主要终产物，表明α-淀粉酶活性。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Extracellular amylase production by the moderate halophile Halomonas meridiana was optimized and the enzyme was characterized biochemically. The highest amylase production was achieved by growing H. meridiana cultures in media with 5% salts and starch, in the absence of glucose until the end of the exponential phase. The amylase exhibited maximal activity at pH 7.0, being relatively stable in alkaline conditions. Optimal temperature and salinity for activity were 37 degrees C and 10%, NaCl, respectively. Moreover, activity at salinity as high as 30% salts was detected. Maltose and maltotriose were the main end products of starch hydrolysis, indicating an alpha-amylase activity. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.