The transcriptional landscape of the mammalian genome
The transcriptional landscape of the mammalian genome
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DOI：
10.1126/science.1112014
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发表时间：
2005-09-02
期刊：
影响因子：
56.9
通讯作者：
Hayashizaki, Y
中科院分区：
文献类型：
--
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
本研究描述了转录起始和终止位点的全面轮询，以及对来自小鼠基因组的先前未识别的全长互补DNA的分析。我们确定了181，047个转录本的5'和3'边界，这些转录本具有由替代启动子使用、剪接和多聚腺苷酸化引起的广泛变异。有16，247个新的小鼠蛋白质编码转录本，包括5154个编码以前未鉴定的蛋白质。转录组的基因组作图揭示了转录森林，在两条链上都有重叠的转录，被沙漠隔开，在沙漠中观察到很少的转录本。这些数据为比较分析哺乳动物分化和发育中的转录调控提供了一个全面的平台。
This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.