Functional and histopathological study of the pancreas in hamsters (Mesocricetus auratus) infected and reinfected with Trypanosoma cruzi
Functional and histopathological study of the pancreas in hamsters (Mesocricetus auratus) infected and reinfected with Trypanosoma cruzi
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DOI：
10.1007/s00436-004-1183-8
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发表时间：
2004-09-01
影响因子：
2
通讯作者：
Ramírez, LE
中科院分区：
文献类型：
--
作者：
dos Santos, VM;de Lima, MA;Ramírez, LE
研究人员对 94 只感染和再感染克氏锥虫 VIC 株的仓鼠以及 73 只未感染的正常对照进行了胰腺组织病理学和功能的变化研究。通过微量血细胞比容、血液培养、特异性过氧化物酶抗过氧化物酶、聚合酶链式反应和血清凝集来验证每只动物的感染。测定血糖和胰岛素。计算每个切片的胰岛数量和抗体标记的胰岛细胞数量。评估了胰岛炎、神经炎、纤维化、萎缩和炎症浸润。实验性恰加斯感染引起的胰腺炎与人类恰加斯病相似，涉及腺泡、胰岛和神经，伴有萎缩和纤维化，尽管与再感染的数量没有相关性。在受感染的动物中观察到血糖水平不稳定和低胰岛素血症的倾向。在急性期，受感染的仓鼠中生长抑素和胰多肽产生细胞的数量较低，这最终与血糖水平的变化和低胰岛素血症有关。我们的研究结果支持存在内分泌形式的慢性恰加斯感染的假设。
Histopathological and functional changes in the pancreas were studied in 94 hamsters infected and reinfected with Trypanosoma cruzi VIC strain and in 73 anon-infected normal controls. Infection in each animal was verified by microhematocrit, hemoculture, specific peroxidase anti-peroxidase, polymerase chain reaction and seroagglutination. Blood glucose and insulin were determined. The number of islets per section and the number of islet cells marked with antibodies were counted. Insulitis, neuritis, fibrosis, atrophy and inflammatory infiltrates were evaluated. Experimental chagasic infection caused pancreatitis similar to human Chagas' disease, involving acini, islets and nerves, with atrophy and fibrosis, although without correlation to the number of reinfections. Erratic blood glucose levels and a tendency to hypoinsulinemia were observed in infected animals. During the acute phase, the number of somatostatin and pancreatic polipeptide producer islet cells was lower in infected hamsters, which was eventually related to changes in blood sugar levels and hypoinsulinemia. Our findings favor the hypothesis of the existence of an endocrine form of chronic chagasic infection.