Expression of E-cadherin and α-catenin in a varicocele-induced infertility rat model
Expression of E-cadherin and α-catenin in a varicocele-induced infertility rat model
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DOI：
10.1038/aja.2010.94
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发表时间：
2011-05-01
影响因子：
2.9
通讯作者：
Park, Nam Cheol
中科院分区：
文献类型：
--
作者：
Ha, Hong Koo;Park, Hyun Jun;Park, Nam Cheol
E-cadherin和α-catenin在精索静脉曲张引起的不孕症的发展中的作用进行了评估。分析E-cadherin/alpha-catenin的表达与临床/病理参数之间的相关性。30只10周龄雄性大鼠（实验组）用于实验;结扎左肾静脉以形成精索静脉曲张。在30只大鼠（对照组）中切开腹部，在10只大鼠（基线组）中不进行手术。分别于造模4周和8周后测定大鼠左侧睾丸重量、血清活性氧（ROS）、睾酮、促黄体生成素（LH）、促卵泡生成素（FSH）水平及曲细精管变性程度。免疫组化染色和Western blot分析E-cadherin和α-catenin的表达。8周实验组的ROS水平较基线组和对照组明显升高（P均< 0.001）。此外，与对照组相比，4周和8周实验组的FSH显著升高（分别为P=0.013和P=0.032）。实验组曲细精管变性改变的比例增加。免疫组化染色显示，实验4周和8周组E-cadherin和alpha-catenin的表达减少。与IHC染色类似，实验组在Western印迹分析中具有降低的反应性。E-cadherin和alpha-catenin的表达与生精小管的ROS和退行性改变显著相关。本研究结果提示，精索静脉曲张引起的男性不育与血睾屏障（BTB）的损伤有关，ROS可能对BTB造成损伤。Asian Journal of Andrology（2011）13，470-475; doi：10.1038/aja.2010.94; 2011年3月14日在线发表
The roles of E-cadherin and alpha-catenin were evaluated in the development of varicocele-induced infertility. Analysis of the association between the expression of E-cadherin/alpha-catenin and clinical/pathological parameters was performed. Thirty 10-week-old male rats (experimental group) were used for the experiments; the left renal vein was ligated to form a varicocele. The abdomen was incised in 30 rats (control group) and no procedure was performed on 10 rats (baseline group). The weights of the left testis, serum reactive oxygen species (ROS), testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and degenerative changes in the seminiferous tubules after 4 and 8 weeks were recorded. The expression of E-cadherin and alpha-catenin was evaluated by immunohistochemical (IHC) staining and Western blot analysis. The ROS increased in the 8-week experimental group, compared with the baseline and control groups (P < 0.001 for both). Additionally, FSH significantly increased in the 4-and 8-week experimental group compared with the control groups (P=0.013 and P=0.032, respectively). The ratio of degenerative changes in the seminiferous tubules of the experimental groups increased. The IHC staining showed that the expression of E-cadherin and alpha-catenin decreased in the 4-and 8-week experimental groups. Similar to the IHC staining, the experimental group had decreased reactivity on Western blot analysis. The expression of E-cadherin and alpha-catenin was significantly associated with the ROS and degenerative changes in the seminiferous tubules. The results of this study suggest that damage to the blood-testis barrier (BTB) is associated with varicocele-induced male infertility, and that ROS may cause damage to the BTB. Asian Journal of Andrology (2011) 13, 470-475; doi:10.1038/aja.2010.94; published online 14 March 2011