Characterization of the MAL2-positive compartment in oligodendrocytes
Characterization of the MAL2-positive compartment in oligodendrocytes
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DOI：
10.1016/j.yexcr.2009.08.003
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发表时间：
2009-11-15
影响因子：
3.7
通讯作者：
Antonio Lopez-Guerrero, Jose
中科院分区：
文献类型：
--
作者：
Bello-Morales, Raquel;de Marco, Maria C.;Antonio Lopez-Guerrero, Jose
少突胶质细胞(OLs)。中枢神经系统产生髓磷脂的细胞在质膜上分离不同的表面亚域，其他分化的细胞如极化上皮和神经元也是如此。在极化上皮中，为了生成具有髓鞘化OLs特征的复杂膜系统，需要合成大量的膜蛋白和脂质并正确靶向。相当一部分的根尖蛋白在被内化并通过胞吞作用穿过细胞到达根尖膜之前，通过迂回到基底膜的间接途径运输。肝细胞的根尖再循环核内体（ARE）或其等效物，即根尖下室（SAC），是细胞内转运途径中的一个细胞内转运站，MAL2是基底侧到根尖胞胞作用机制的重要组成部分，是一种ARE/SAC驻留蛋白。在这里，我们显示，分化后，小鼠少突胶质细胞前体和人类少突胶质细胞衍生细胞系。在这些少突细胞系中，Oli-neu和HOG分别上调MAL2的表达，并将其积聚在细胞内腔室中，表现出中心体周围的定位。该隔间共享ARE/SAC的一些主要功能，例如与Rab11a共定位。因此，我们认为少突胶质细胞中的mal2阳性隔室可能是一种类似于ARE/SAC的结构，并且可能在少突胶质细胞分化过程中对髓磷脂组装的蛋白质和脂质进行分拣中起重要作用(C) 2009 Elsevier Inc.)。版权所有
Oligodendrocytes (OLs). the myelin-producing cells of the central nervous system, segregate different surface subdomains at the plasma membrane as do other differentiated cells Such as polarized epithelia and neurons. To generate the complex membrane system that characterizes myelinating OLs, large amounts of membrane proteins and lipids need to be synthesized and correctly targeted In polarized epithelia. a considerable fraction of apical proteins are transported by an indirect pathway involving a detour to the basolateral membrane before being internalized and transported across the cell to the apical membrane by a process known as transcytosis. The apical recycling endosome (ARE) or its equivalent, the subapical compartment (SAC), of hepatocytes is an intracellular trafficking station involved in the transcytotic pathway MAL2, an essential component of the machinery for basolateral-to-apical transcytosis, is an ARE/SAC resident protein. Here, we show that, after differentiation, murine oligodendrocyte precursor and human oligodendroglioma derived cell lines. Oli-neu and HOG, respectively, up-regulate the expression of MAL2 and accumulate it in an intracellular compartment, exhibiting a peri-centrosomal localization In these oligodendrocytic cell lines. this compartment shares some of the main features of the ARE/SAC, such as colocalization with Rab11a. sensitivity to disruption of the microtubule cytoskeleton with nocodazole, and lack of internalized transferrin Therefore, we Suggest that the MAL2-positive compartment in oligodendrocytic cells could be a structure analogous to the ARE/SAC and might have an important role in the sorting of proteins and lipids for myelin assembly during oligodendrocyte differentiation (C) 2009 Elsevier Inc. All rights reserved