Analytical possibilities for the detection of stanozolol and its metabolites
Analytical possibilities for the detection of stanozolol and its metabolites
复制标题
DOI：
10.1016/s0003-2670(02)00672-4
复制
发表时间：
2002-11-25
影响因子：
6.2
通讯作者：
Van Peteghem, C
中科院分区：
文献类型：
--
作者：
Poelmans, S;De Wasch, K;Van Peteghem, C
在体育兴奋剂以及人类和赛马中，斯坦诺唑尔（Stan）被滥用，并成为代谢研究的主题。在兽医实践中，斯坦诺唑尔也成为一种重要的误用合成代谢类固醇。像大多数其他合成代谢类固醇一样，斯坦诺唑尔的气相色谱行为很差。由于尿排泄和肾脏清除率低，在尿液中很难检测到。这是由于快速代谢，导致母体化合物在尿液中发现的低浓度水平。因此，大多数研究都集中在其尿液代谢物的检测上。对于代谢物的鉴定，文献中描述了不同的提取和检测方法。本文将对此进行回顾。大多数作者使用水解来释放II期代谢物。提取方法有固相萃取（SPE）、液-液萃取（L-L）和免疫亲和层析（IAC）。对于最终的检测，采用气相色谱(GC)-质谱（MS）可与液相色谱(LC)-MSn相比较。在动物实验中，根据施替诺唑尔的剂量（口服或肌肉注射），鉴定出不同的代谢物。对这些分析物在其他基质中的分析也作了简要讨论。(C) 2002 Elsevier Science B.V.版权所有
In sports doping, as well in man as in horseracing, stanozolol (Stan) was abused and became the subject of metabolism research. Also in veterinary practice, stanozolol became an important misused anabolic steroid. Like most other anabolic steroids, stanozolol has poor gas chromatographic behavior. It is difficult to detect in urine, because of low urinary excretion and renal clearance. This is due to the rapid metabolization, leading to low concentration levels of the parent compound found in urine. Therefore, most research studies have focused on the detection of its urinary metabolites. For the identification of the metabolites, different methods of extraction and detection are described in the literature. These are reviewed in this article. Most authors use a hydrolysis to free the phase II metabolites. Extraction procedures vary from solid-phase extraction (SPE), liquid-liquid (L-L) extraction to immunoaffinity chromatography (IAC). For the final detection, the use of gas chromatography (GC)-mass spectrometry (MS) can be compared with liquid chromatography (LC)-MSn. Different metabolites are identified depending on the administration of stanozolol in the animal experiment (oral or intramuscular). Analyses for these analytes in other matrices are also briefly discussed. (C) 2002 Elsevier Science B.V. All rights reserved.