The high-pathogenicity island of Yersinia pseudotuberculosis can be inserted into any of the three chromosomal asn tRNA genes
The high-pathogenicity island of Yersinia pseudotuberculosis can be inserted into any of the three chromosomal asn tRNA genes
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DOI：
10.1046/j.1365-2958.1998.01124.x
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发表时间：
1998-12-01
影响因子：
3.6
通讯作者：
Carniel, E
中科院分区：
文献类型：
--
作者：
Buchrieser, C;Brosch, R;Carniel, E
已在几种细菌中发现了致病岛(PAI)，在小肠结肠炎耶尔森菌和鼠疫耶尔森菌中已发现携带铁获取相关基因的高致病力岛(HPI)。本研究对第三种人类致病耶尔森菌假结核耶尔森菌的HPI进行了表征，证明了IP32637菌株的HPI具有与鼠疫耶尔森菌相同的物理和遗传图谱，与噬菌体P4整合酶基因同源的基因位于IP32637株HPI的ASN tRNA位点下游。这个int基因在所有三种致病耶尔森氏菌的HPI上都处于相同的位置。然而，与鼠疫杆菌6/69相比，假结核杆菌IP32637的HPI并不总是与色素片段相邻，并且可以插入到该片段的大于或等于190kb的距离；与鼠疫杆菌和小肠结肠炎耶尔森菌相比，假结核杆菌IP32637的HPI可以准确地从染色体上切除，并且引人注目的是，它可以插入到该物种染色体上存在的三个ASN tRNA基因座中的任何一个，其中一个与色素片段相邻，即色素片段，该色素片段存在于鼠疫菌中而不是在小肠结肠炎耶尔森菌中。在所有研究过的假结核耶尔森菌株中也存在并且保存良好。相反，HPI的存在和大小因菌株的血清型而异：仅在I型菌株中发现完整的HPI，携带IS100序列的左侧部分有9kb截断的HPI，而III型菌株没有发现HPI，而II型、IV型和V型菌株则没有发现HPI。
Pathogenicity islands (PAIs) have been identified in several bacterial species, A PAI called high-pathogenicity island (HPI) and carrying genes involved in iron acquisition (yersiniabactin system) has been previously identified in Yersinia enterocolitica and Yersinia pestis, In this study, the HPI of the third species of Yersinia pathogenic for humans, Y. pseudotuberculosis, has been characterized, We demonstrate that the HPI of strain IP32637 has a physical and genetic map identical to that of Y. pestis, A gene homologous to the bacteriophage P4 integrase gene is located downstream of the asn tRNA locus that borders the HPI of strain IP32637, This int gene is at the same position on the HPI of all three pathogenic Yersinia species. However, in contrast to Y. pestis 6/69, the HPI of Y. pseudotoberculosis IP32637 is not invariably adjacent to the pigmentation segment and can be inserted at a distance greater than or equal to 190 kb from this segment, Also, in contrast to Y. pestis and Y,enterocolitica, the HPI of Y. pseudotuberculosis IP32637 can precisely excise from the chromosome, and, strikingly, it can be found inserted in any of the three asn tRNA loci present on the chromosome of this species, one of which is adjacent to the pigmentation segment, The pigmentation segment, which is present in Y. pestis but not in Y. enterocolitica, is also present and well conserved in all strains of Y. pseudotuberculosis studied. In contrast, the presence and size of the HPIs vary depending on the serotype of the strain: an entire HPI is found in strains of serotypes I only, a HPI with a 9 kb truncation in its left-hand part that carries the IS100 sequence and the psn and ybtE genes characterizes the strains of serotype III, and no HPI is found in strains of serotypes II, IV and V.