Development and characterization of species-diagnostic ISSR and SCAR DNA markers for differentiating red maple (Acer rubrum) and silver maple (A. saccharinum)
Development and characterization of species-diagnostic ISSR and SCAR DNA markers for differentiating red maple (Acer rubrum) and silver maple (A. saccharinum)
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DOI：
10.1139/gen-2019-0037
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发表时间：
2019-08-01
期刊：
影响因子：
3.1
通讯作者：
Nkongolo, Kabwe K.
中科院分区：
文献类型：
--
作者：
Boyd, Meagan;Panoyan, Mary Anne;Nkongolo, Kabwe K.
红枫（Acer rubrum）和银枫（A. saccharinum）是在自然界中容易杂交的姊妹种。未在这些种属中检测遗传或条形码标记。本研究的主要目的是开发和表征用于区分A. rubrum和A. saccharinum的杂交，并验证A. freemanii是利用ISSR标记系统从它们的杂交中获得的。十三A rubrum和7个A.使用糖精群体。选用4个ISSR引物（ISSR5、ISSR8、ISSR10和ISSRUBC 825）对两种野生稻及其杂种的基因组DNA进行扩增。每个引物产生至少一个物种诊断的ISSR标记，共六个。对A. freemanii的杂交结果证实，所筛选的基因型为A. rubrum和A.糖精。这些标记被克隆和测序。使用专门设计的引物将成功的序列转化为SCAR标记。总体而言，开发的诊断和特异性的ISSR和SCAR标记是有用的，在这两个枫树物种及其杂交种的认证。它们可用于跟踪A. rubrum和A. saccharinum DNA在其他杂交树或种群中的表达。
Red maple (Acer rubrum) and silver maple (A. saccharinum) are sister species that readily hybridize in nature. No genetic or barcoding markers have been tested in these species. The main objective of the present study is to develop and characterize molecular markers for distinguishing A. rubrum and A. saccharinum and to validate the hybridity of A. freemanii derived from their crossings using the ISSR marker system. Thirteen A. rubrum and seven A. saccharinum populations were used. Four ISSR primers including ISSR 5, ISSR 8, ISSR 10, and ISSR UBC 825 were selected to amplify genomic DNA from the two species and their hybrids. Each primer generated at least one species-diagnostic ISSR marker for a total of six. Analysis of A. freemanii collected from North Dakota (USA) confirmed that the genotypes screened were true hybrids between A. rubrum and A. saccharinum. These markers were cloned and sequenced. Successful sequences were converted to SCAR markers using specifically designed primers. Overall, the developed diagnostic and specific ISSR and SCAR markers are useful in the certification of these two maple species and their hybrids. They can be used in tracking the introgression of A. rubrum and A. saccharinum DNA in other hybrid trees or populations.