The influence of tethered epidermal growth factor on connective tissue progenitor colony formation
The influence of tethered epidermal growth factor on connective tissue progenitor colony formation
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DOI：
10.1016/j.biomaterials.2009.05.061
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Griffith, Linda G.
中科院分区：
文献类型：
--
作者：
Marcantonio, Nicholas A.;Boehm, Cynthia A.;Griffith, Linda G.
将联合收割机抽吸的骨髓细胞与支架结合以治疗结缔组织缺损的策略正获得越来越多的临床关注和使用。在诸如大缺陷的情况下，移植的结缔组织祖细胞（CTP）的初始存活和增殖受到限制，通过使用支架递送促进移植物中细胞功能的早期阶段的生长因子，可以改善治疗结果。表皮生长因子受体（EGFR）的信号传导在细胞存活中起作用，并与骨发育和体内平衡有关。与可溶性配体相比，以支架栓系形式提供表皮生长因子（EGF）可以维持局部递送并将EGFR信号传导转变为促存活模式。因此，我们研究了拴系EGF对成骨细胞集落形成的影响，从人骨髓穿刺液在三种不同的粘附环境中，共使用39个捐助者。我们发现，栓系EGF，但不可溶性EGF，增加了无论粘连背景的集落形成的数量，栓系EGF不损害早期阶段的成骨分化。(C)2009爱思唯尔有限公司保留所有权利。
Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation. (C) 2009 Elsevier Ltd. All rights reserved.