Expression of mRNA encoding the H1, H2, and H3 histamine receptors in the rat cochlea
Expression of mRNA encoding the H1, H2, and H3 histamine receptors in the rat cochlea
复制标题
大鼠耳蜗中编码 H1、H2 和 H3 组胺受体的 mRNA 表达
DOI：
10.1097/00001756-200303030-00025
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发表时间：
2003
期刊：
影响因子：
1.7
通讯作者：
T. Takeda
中科院分区：
文献类型：
--
作者：
H. Azuma;S. Sawada;S. Takeuchi;K. Higashiyama;A. Kakigi;T. Takeda
组胺可能在内耳中具有生理功能。然而，哺乳动物耳蜗中尚未发现组胺受体。我们使用逆转录聚合酶链反应 (RT-PCR) 确定 H1、H2 和 H3 组胺受体 mRNA 在大鼠耳蜗中表达。为了研究每种受体的分布，耳蜗被分为三个部分：外侧部分、内侧部分和蜗轴。每种受体亚型的 mRNA 在耳蜗轴中检测到，但在耳蜗的外侧和内侧部分未检测到。这是大鼠耳蜗中存在 H1、H2 和 H3 组胺受体 mRNA 的第一个证据。这些发现支持组胺可能在耳蜗中发挥生理作用的建议。
Histamine may have physiological functions in the inner ear. Histamine receptors, however, have not yet been identified in the mammalian cochlea. We determined, using reverse transcription polymerase chain reaction (RT-PCR), that H1, H2, and H3 histamine receptor mRNA is expressed in the rat cochlea. To investigate the distribution of each receptor, the cochlea was divided into three parts: the lateral portion, the medial portion, and the modiolus. The mRNA for each receptor subtype was detected in the modiolus but not in the lateral and medial portions of the cochlea. This is the first evidence of H1, H2, and H3 histamine receptor mRNA in the rat cochlea. These findings support the suggestion that histamine may play a physiological role in the cochlea.