Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments
Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments
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DOI：
10.1039/c1lc20147f
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Wo, Andrew M.
中科院分区：
文献类型：
--
作者：
Hsiung, Lo-Chang;Chiang, Chi-Ling;Wo, Andrew M.
我们提出了一种基于介电泳法(DEP)的细胞微阵列芯片，用于在灌流微环境中基于细胞的抗癌药物筛选。将人乳腺癌细胞MCF7种植到芯片中，并通过DEP力将其图案化到平面交指环形电极(PERO)阵列上。粗略地说，芯片只需要三分之一的细胞数量，而96孔板对照则需要。药物浓度(顺铂或多西紫杉醇)通过浓度梯度发生器(CGG)和抗串扰阀(ACV)的功能集成稳定地产生，以处理细胞24小时。用双染色法对细胞活性进行定量。细胞图案化的结果显示图案化的细胞基本均匀(每层92+/-5个细胞)。此外，在药物灌流24小时后，芯片和96孔板对照组之间的剂量反应没有统计学意义。芯片的IC50值也与文献中的值一致。此外，灌流培养显示了同一小室中不同PIre上细胞药物反应的重复性。该芯片将使细胞供应有限的应用成为可能，并为临床实践补充微流控灌流培养。
We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 +/- 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose-responses between the chip and the 96-well plate controls was found. The IC50 value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices.