A Novel Microchip Technique for Quickly Identifying Nanogranules in an Aqueous Solution by Transmission Electron Microscopy: Imaging of Platelet Granules
A Novel Microchip Technique for Quickly Identifying Nanogranules in an Aqueous Solution by Transmission Electron Microscopy: Imaging of Platelet Granules
复制标题
通过透射电子显微镜快速识别水溶液中纳米颗粒的新型微芯片技术：血小板颗粒成像
作者：
N. Trang;Jungshan Chang;Wei;Chih;Huixian Chen;Chao;T. Fong
生物样品或纳米颗粒的超微结构观察通常需要使用电子显微镜。电子显微镜需要大量的时间，需要许多步骤，并使用许多化学品，这可能会影响生物标本的天然状态。一种新型的微芯片（K-套件）被用作标本盒，用于在水溶液中使用透射电子显微镜的人血小板颗粒的原位成像。这种微芯片使我们能够非常快速和容易地观察天然的人类血小板颗粒。实验过程包括采血、血小板纯化、血小板颗粒分离、样品上样、透射电镜观察。此外，这些颗粒仍然可以保留在水溶液中，并且仅需要非常少量的样品用于观察和分析。我们使用这种微芯片通过负染色来识别天然血小板颗粒。此外，我们使用该芯片进行免疫电镜，并成功地标记血小板α颗粒与抗P-选择素抗体。这些结果表明，这种新型微芯片可以为研究人员提供更快，更好的选择，当使用透射电子显微镜在水性条件下检查生物样本的纳米颗粒时。
Ultrastructural observation of biological specimens or nanogranules usually requires the use of electron microscopy. Electron microscopy takes a lot of time, requires many steps, and uses many chemicals, which may affect the native state of biological specimens. A novel microchip (K-kit) was used as a specimen kit for in situ imaging of human platelet granules in an aqueous solution using a transmission electron microscope. This microchip enabled us to observe the native human platelet granules very quickly and easily. The protocols included blood collection, platelet purification, platelet granule isolation, sample loading into this microchip, and then observation by a transmission electron microscope. In addition, these granules could still remain in aqueous solution, and only a very small amount of the sample was required for observation and analysis. We used this microchip to identify the native platelet granules by negative staining. Furthermore, we used this microchip to perform immunoelectron microscopy and successfully label α-granules of platelets with the anti-P-selectin antibody. These results demonstrate that the novel microchip can provide researchers with faster and better choices when using a transmission electron microscope to examine nanogranules of biological specimens in aqueous conditions.
影响因子：
20.3
作者：
Christian G Peters;A. Michelson;R. Flaumenhaft
通讯作者：
Christian G Peters;A. Michelson;R. Flaumenhaft
影响因子：
16
作者：
Nogales, Eva;Scheres, Sjors H. W.
通讯作者：
Scheres, Sjors H. W.
人类血小板囊泡表现出不同的大小和蛋白质组。
影响因子：
4.4
作者：
Jena,BhanuP;Stemmer,PaulM;Wang,Sunxi;Mao,Guangzhao;Lewis,KennethT;Walz,DanielA
通讯作者：
Walz,DanielA