Production and Tolerance of Ethanol in Relation to Phospholipid Fatty-acyl Composition in Saccharomyces cerevisiae NCYC 431
Production and Tolerance of Ethanol in Relation to Phospholipid Fatty-acyl Composition in Saccharomyces cerevisiae NCYC 431
复制标题
酿酒酵母 NCYC 431 中乙醇的产生和耐受性与磷脂脂酰基组成的关系
DOI：
10.1099/00221287-128-7-1447
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发表时间：
1982
期刊：
影响因子：
1.5
通讯作者：
A. H. Rose
中科院分区：
文献类型：
--
作者：
Michael J. Beaven;C. Charpentier;A. H. Rose
总结：从厌氧培养的酿酒酵母NCYC 431的上清液中乙醇的积累密切抑制生长在早期的指数阶段的分批生长，并继续生长后停止。在发酵的8-64 h期间，细胞内乙醇浓度大于细胞外浓度。通过用水洗涤，可以非常快速地从生物体中提取乙醇。在生长过程中长达32小时，有一个渐进的减少脂肪酰基不饱和磷脂，和相应的比例增加饱和度。此后，这一趋势略有逆转。培养8小时后，补充乙醇（0.5或1.0 M）的培养物延缓生长速率，而补充1.5 M-乙醇立即停止生长。在培养补充0.5或1.0 m-乙醇，活力没有降低，但补充1.5 m-乙醇引起活力迅速下降。在任何三个浓度的乙醇补充培养物导致细胞磷脂中的单不饱和脂肪酰基残基的比例增加，特别是在C18残基，这是伴随着饱和残基的比例下降。
Summary: Accumulation of ethanol in supernatants from anaerobic cultures of Saccharomyces cerevisiae NCYC 431 closely paralleled growth during the early exponential phase of batch growth, and continued after growth had ceased. During the 8–64 h period of the fermentation, the intracellular ethanol concentration was greater than the extracellular concentration. Ethanol was very rapidly extracted from organisms by washing with water. During growth up to 32 h, there was a progressive decrease in fatty-acyl unsaturation in phospholipids, and a corresponding proportional increase in saturation. Thereafter, the trend was very slightly reversed. Supplementing cultures with ethanol (0.5 or 1.0m) after 8h incubation retarded growth rate, while supplementation with 1.5 m-ethanol immediately stopped growth. In cultures supplemented with 0.5 or 1.0 m-ethanol, viability was not lowered, but supplementation with 1.5 m-ethanol caused a rapid decline in viability. Supplementation of cultures with ethanol at any of the three concentrations led to an increase in the proportion of mono-unsaturated fatty-acyl residues in cellular phospholipids, especially in C18 residues, which was accompanied by a decrease in the proportion of saturated residues.
微生物糖酵解的生化遗传学。
DOI：
10.1007/978-1-4684-3980-9_13
发表时间：
1981
期刊：
Basic life sciences
影响因子：
--
作者：
Fraenkel,DG
通讯作者：
Fraenkel,DG