Simplified procedure for the quantitation of radioactive phosphoinositides by thin-layer chromatography.
Simplified procedure for the quantitation of radioactive phosphoinositides by thin-layer chromatography.
复制标题
通过薄层色谱法定量放射性磷酸肌醇的简化程序。
DOI：
10.1016/s0021-9673(01)90415-7
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Dale,GL
中科院分区：
文献类型：
--
作者：
Crosby,SD;Dale,GL
MATERIALS AND METHODSSilica gel 60 thin-layer plates, 0.25 mm thickness with preadsorbent spotting area were purchased from Merck (Darmstadt, FRG) and JT Baker (Phillipsburg, NJ, USA). Aerosol power cans (Chromist) were from Gelman (Ann Arbor, MI, USA). All solvents were of reagent grade. 32P-Labeled lipids from human erythrocytes were prepared and isolated according to Ferrell and Huestis 4. Lipids were separated by chromatography in chloroform-methanol-3.3 N ammonium hydroxide (75: 60: 19) 4.
