Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing
Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing
复制标题
DOI：
10.3390/microorganisms8040485
复制
发表时间：
2020-04-01
期刊：
影响因子：
4.5
通讯作者：
Sacchetti, Lucia
中科院分区：
文献类型：
--
作者：
Nardelli, Carmela;Granata, Ilaria;Sacchetti, Lucia
肠道菌群可能对肥胖有影响。迄今为止，大多数对肥胖患者的研究报告了粪便样本中的微生物群组成。本研究的目的是调查意大利坎帕尼亚地区肥胖人群比例很高的成年肥胖者的十二指肠黏膜失调，以突出可能与肥胖相关的微生物分类群。对19例肥胖者（OB）和16例瘦子对照组（CO）在上消化道内镜检查时进行十二指肠活检，并对微生物组进行16S rRNA基因测序。各组十二指肠微生物群包括变形菌门、厚壁菌门、放线菌门、梭菌门、拟杆菌门和酸杆菌门6门。OB中变形菌门（51.1%比40.1%）和厚壁菌门（33.6%比44.9%）的丰度显著高于CO (p < 0.05)；与CO相比，OB中嗜糖Oribacterium asaccharolyticum， parvulum和Fusobacterium nucleatum减少（p < 0.01）， Pseudomonadales增加（p < 0.05）。受试者工作特征曲线分析显示，Atopobium和Oribacterium属能够准确区分OB和CO（分别为75%和78%）。由此可见，肥胖受试者的十二指肠微生物群中变形菌群增加，厚壁菌门（Lachnospiraceae）减少。这些数据为进一步的研究提供了指导，以评估与生态失调肥胖相关的信号的功能作用。
The gut microbiota may have an impact on obesity. To date, the majority of studies in obese patients reported microbiota composition in stool samples. The aim of this study was to investigate the duodenal mucosa dysbiosis in adult obese individuals from Campania, a region in Italy with a very high percentage of obese people, to highlight microbial taxa likely associated with obesity. Duodenum biopsies were taken during upper gastrointestinal endoscopy in 19 obese (OB) and 16 lean control subjects (CO) and microbiome studied by 16S rRNA gene sequencing. Duodenal microbiome in our groups consisted of six phyla: Proteobacteria, Firmicutes, Actinobacteria, Fusobacteria, Bacteroidetes and Acidobacteria. Proteobacteria (51.1% vs. 40.1%) and Firmicutes (33.6% vs. 44.9%) were significantly (p < 0.05) more and less abundant in OB compared with CO, respectively. Oribacterium asaccharolyticum, Atopobium parvulum and Fusobacterium nucleatum were reduced (p < 0.01) and Pseudomonadales were increased (p < 0.05) in OB compared with CO. Receiver operating characteristic curve analysis showed Atopobium and Oribacterium genera able to discriminate with accuracy (power = 75% and 78%, respectively) OB from CO. In conclusion, increased Proteobacteria and decreased Firmicutes (Lachnospiraceae) characterized the duodenal microbiome of obese subjects. These data direct to further studies to evaluate the functional role of the dysbiotic-obese-associated signature.