Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans
Identification of Specific Nuclear Genetic Loci and Genes That Interact With the Mitochondrial Genome and Contribute to Fecundity in Caenorhabditis elegans
复制标题
鉴定与线粒体基因组相互作用并有助于秀丽隐杆线虫繁殖力的特定核遗传位点和基因
DOI：
10.3389/fgene.2019.00028
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发表时间：
2019-02-04
影响因子：
3.7
通讯作者：
Han，Conghui
中科院分区：
文献类型：
--
作者：
Zhu，Zuobin;Han，Xiaoxiao;Han，Conghui
先前的研究发现，生殖力是一个多基因性状，部分地受到细胞核（mit-n）遗传互作的调控。然而，确定特定的核遗传位点或基因与线粒体基因组相互作用，并有助于数量性状繁殖是一个悬而未决的问题。本研究利用C.C.N2和CB4856菌株杂交建立了一组重组自交高级互交系（RIAIL）。elegans，被用来描述与生殖力相关的mit-n遗传相互作用的潜在遗传基础。在与有丝分裂型连锁的115个单核苷酸多态性（SNPs）中，通过关联作图，发现67个SNPs与生殖力相关。这表明核遗传和线粒体遗传对繁殖力有显著的上位效应。此外，两个特定的核遗传位点与线粒体基因组相互作用，并有助于繁殖力被确定。在携带CB4856线粒体和N2基因型在基因座1或CB4856基因型在基因座2相对于野生型菌株的RIAILs中观察到繁殖力的显着降低。然后，建立了一个杂交菌株（CNC10），它是在CB4856核背景下培育的，具有CB4856 mtDNA基因组的同质性和第1位点的N2基因型。CNC10的平均繁殖力是对照菌株的一半。CNC10线粒体的几个功能特征也受到mit-n相互作用的影响。总体而言，实验证据表明，特定的核遗传位点或基因与线粒体基因组相互作用，并与繁殖力。总共有18个基因被确定使用整合的方法与线粒体基因组的相互作用，并有助于繁殖力。
Previous studies have found that fecundity is a multigenic trait regulated, in part, by mitochondrial-nuclear (mit-n) genetic interactions. However, the identification of specific nuclear genetic loci or genes interacting with the mitochondrial genome and contributing to the quantitative trait fecundity is an unsolved issue. Here, a panel of recombinant inbred advanced intercrossed lines (RIAILs), established from a cross between the N2 and CB4856 strains of C. elegans, were used to characterize the underlying genetic basis of mit-n genetic interactions related to fecundity. Sixty-seven single nucleotide polymorphisms (SNPs) were identified by association mapping to be linked with fecundity among 115 SNPs linked to mitotype. This indicated significant epistatic effects between nuclear and mitochondria genetics on fecundity. In addition, two specific nuclear genetic loci interacting with the mitochondrial genome and contributing to fecundity were identified. A significant reduction in fecundity was observed in the RIAILs that carried CB4856 mitochondria and a N2 genotype at locus 1 or a CB4856 genotype at locus 2 relative to the wild-type strains. Then, a hybrid strain (CNC10) was established, which was bred as homoplasmic for the CB4856 mtDNA genome and N2 genotype at locus 1 in the CB4856 nuclear background. The mean fecundity of CNC10 was half the fecundity of the control strain. Several functional characteristics of the mitochondria in CNC10 were also influenced by mit-n interactions. Overall, experimental evidence was presented that specific nuclear genetic loci or genes have interactions with the mitochondrial genome and are associated with fecundity. In total, 18 genes were identified using integrative approaches to have interactions with the mitochondrial genome and to contribute to fecundity.