RESPONSE OF RESTING HUMAN PERIPHERAL-BLOOD NATURAL-KILLER CELLS TO INTERLEUKIN-2
RESPONSE OF RESTING HUMAN PERIPHERAL-BLOOD NATURAL-KILLER CELLS TO INTERLEUKIN-2
复制标题
DOI：
10.1084/jem.160.4.1147
复制
发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
PERUSSIA, B
中科院分区：
文献类型：
--
作者：
TRINCHIERI, G;MATSUMOTOKOBAYASHI, M;PERUSSIA, B
重组人白细胞介素2（IL-2）经纯化后，能迅速增强人外周血淋巴细胞的自发性细胞毒活性。用IL-2处理18小时后介导细胞毒性的细胞具有自然杀伤（NK）细胞的表面标志物，并且从含有自发细胞毒性细胞的外周血亚群产生。γ的并行产生干扰素（IFN-γ）由重组IL-2（rIL-2）诱导，并且NK细胞似乎是主要的生产细胞，而T细胞不能产生IFN-γ。在这些实验条件下。细胞毒性增强的动力学比IFN-γ的那些更快。生产和单克隆抗IFN-γ。抗体不抑制这种作用，使得IFN-γ不太可能抑制这种作用。生产是负责增强。由rIL-2诱导的NK细胞活性的增强先于淋巴细胞的任何增殖反应，而这在NK和T细胞的长期培养物中观察到。
Recombinant interleukin 2 (IL-2) purified to homogeneity apparently induces a rapid and potent enhancement of spontaneous cytotoxicity of human peripheral blood lymphocytes. The cells mediating cytotoxicity after 18 h treatment with IL-2 have surface markers of natural killer (NK) cells and are generated from the peripheral blood subset containing spontaneous cytotoxic cells. A parallel production of .gamma. interferon (IFN-.gamma.) is induced by recombinant IL-2 (rIL-2), and NK cells appear to be the major producer cells, whereas T cells are unable to produce IFN-.gamma. under these experimental conditions. The kinetics of the enhancement of cytotoxicity are faster than those of IFN-.gamma. production, and monoclonal anti-IFN-.gamma. antibodies do not suppress this effect, making it unlikely that the IFN-.gamma. produced is responsible for the enhancement. The enhancement of NK cell activity induced by rIL-2 precedes any proliferative response of the lymphocytes, which is instead observed in longer-term cultures of NK and T cells.