AEROBIC GLYCOLYSIS IN BONE - LACTIC-ACID PRODUCTION BY RAT CALVARIA CELLS IN CULTURE
AEROBIC GLYCOLYSIS IN BONE - LACTIC-ACID PRODUCTION BY RAT CALVARIA CELLS IN CULTURE
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DOI：
10.1152/ajpcell.1978.234.1.c51
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发表时间：
1978-01-01
影响因子：
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通讯作者：
FLEISCH, H
中科院分区：
文献类型：
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作者：
FELIX, R;NEUMAN, WF;FLEISCH, H
关于完整骨标本的有氧糖酵解已经积累了相当多的数据。为了测试有氧糖酵解是否是骨细胞本身或完整骨内局部条件的特征，培养从大鼠颅骨分离的细胞，并确定几种因素对乳酸产生的影响。当培养基的pH值降低时，这些细胞的乳酸盐产量急剧下降，pH值从7.4变为6.75时，乳酸盐产量从100%变为20%。L-乳酸在20 mM时抑制其自身形成40%。甲状旁腺激素（PTH）（820 U/mg）在0.2至5.0 U/ml的浓度范围内轻微刺激乳酸产生的对数线性响应，处理对照的比例从1.1变化到1.3。在pH 7.0时观察到最大刺激。分离的细胞的定性反应与完整的颅骨相同。在数量上，有显着差异：值得注意的是，甲状旁腺激素的反应较小，对pH值降低的敏感性较高。
Considerable data have been accumulated on aerobic glycolysis of intact bone preparations. To test whether aerobic glycolysis is a feature of the bone cells themselves or of the localized conditions within an intact bone, cells isolated from rat calvaria were cultured and the effect of several factors on lactate production was determined. These cells drastically decreased lactate production when the pH in the culture medium was lowered, changing from 100 to 20 percent for a pH shift from 7.4 to 6.75. L-lactate inhibited its own formation by 40 percent at 20 mM. Parathyroid hormone (PTH) (820 U/mg) at a concentration ranging from 0.2 to 5.0 U/ml stimulated slightly the lactate production in a log-linear response, the ratio treated over control changing from 1.1 to 1.3. The maximal stimulation is observed at pH 7.0. Isolated cells respond qualitatively the same as did intact calvaria. Quantitatively, there were significant differences: notably, a smaller response to parathyroid hormone and a higher sensitivity to lowered pH.