Effects of high temperature on starch morphology and the expression of genes related to starch biosynthesis and degradation
Effects of high temperature on starch morphology and the expression of genes related to starch biosynthesis and degradation
复制标题
高温对淀粉形态及淀粉生物合成与降解相关基因表达的影响
DOI：
10.1016/j.jcs.2016.11.005
复制
发表时间：
2017-01-01
影响因子：
3.8
通讯作者：
Li, Cheng
中科院分区：
文献类型：
--
作者：
Li, Chun Yan;Zhang, Run Qi;Li, Cheng
高温是小麦籽粒灌浆过程中的常见现象。本研究的目的是研究花后高温对淀粉粒形态和淀粉合成与降解相关基因转录的影响。小麦植物在24/17摄氏度（对照）和37/28摄氏度（高温）的昼夜温度下从开花后5天生长，直到籽粒干物质积累停止。在暴露于高温时，成熟的籽粒经历显著的收缩，并且颗粒表面上的点蚀的迹象更加明显。高温改变了淀粉生物合成过程的时间，导致淀粉生物合成过程中基因表达的高峰提前。灌浆期高温对籽粒α-淀粉酶活性有明显的促进作用，淀粉粒易被酶水解。这一结果支持了这一假设，即颗粒表面的点蚀增加与暴露于开花后高温的小麦籽粒中α-淀粉酶活性增强有关。(C)2016由Elsevier Ltd.出版
High temperature occurs frequently during grain filling in wheat (Triticum aestivum L.). The objective of this study was to investigate the high-temperature post anthesis effect on the starch granule morphology and gene transcripts that are involved in starch biosynthesis and degradation. Wheat plants were grown under day night temperatures of 24/17 degrees C (control) and 37/28 degrees C (high temperature) from 5 days post anthesis until kernel dry matter accumulation ceased. Upon exposure to high temperature, the matured kernels underwent significant shrinkage, and signs of pitting on the granule surface were more pronounced. The high temperature altered the timing of the starch biosynthetic process and resulted in an earlier peak in the gene expression during starch biosynthesis. The high temperature had a distinct effect on the enhancement of the grain alpha-amylase activity during kernel filling, and the starch granules were susceptible to enzymatic hydrolysis. This result supported the hypothesis that increased pitting of the granule surface is related to an enhanced alpha-amylase activity in wheat kernels that are exposed to high temperature post anthesis. (C) 2016 Published by Elsevier Ltd.