Characterization of the DNA adenine 5′-GATC-3′ methylase HpyIIIM from Helicobacter pylori
Characterization of the DNA adenine 5′-GATC-3′ methylase HpyIIIM from Helicobacter pylori
复制标题
DOI：
10.1007/s00284-004-4244-4
复制
发表时间：
2004-07-01
影响因子：
2.6
通讯作者：
Taylor, DE
中科院分区：
文献类型：
--
作者：
Simala-Grant, JL;Lam, E;Taylor, DE
幽门螺杆菌（H. pylori）对错配修复、粘附和体外适应性的影响。90 H染色体DNA。pylori菌株，并且限制性内切酶消化表明所有检测的菌株都具有HpyIIIM。野生型H. pylori和一个HpyIIIM失活的菌株的利福平突变频率分别为2.93 × 10 - 7和1.05 × 10 - 7（P > 0.05），表明HpyIIIM在错配修复中似乎并不重要。坚持H。幽门螺杆菌在体外模型细胞系统中也不受HpyIIIM失活的影响。HpyIIIM的失活并没有导致健身的减少，液体在体外竞争实验中确定。
The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylori and a strain with an inactive HpyIIIM were found to have rifampicin mutation frequencies of 2.93 x 10(-7) and 1.05 x 10(-7) (P > 0.05), respectively, indicating that HpyIIIM does not appear to be important in mismatch repair. Adherence of H. pylori in an in vitro model cell system was also unaffected by inactivation of HpyIIIM. Inactivation of HpyIIIM did not result in a decrease in fitness, as determined by liquid in vitro competition experiments.