Female sex pheromone of Athetis lepigone (Lepidoptera: Noctuidae): Identification and field evaluation
Female sex pheromone of Athetis lepigone (Lepidoptera: Noctuidae): Identification and field evaluation
复制标题
斑蛾雌性性信息素（鳞翅目：夜蛾科）的鉴定与田间评价
DOI：
10.1111/jen.12413
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发表时间：
2018
影响因子：
1.9
通讯作者：
Zhang Ya-Nan
中科院分区：
文献类型：
--
作者：
Yan Qi;Zheng Mei-Yan;Xu Ji-Wei;Ma Ji-Fang;Chen Yan;Dong Zhi-Ping;Liu Lei;Dong Shuang-Lin;Zhang Ya-Nan
欧洲和亚洲的许多国家都有记录到白斑潜蝇，但直到 2005 年才被记录为农业害虫。为了利用性信息素来控制这种害虫，我们进行了一项研究，通过触角电检测器气相色谱法 (GC-EAD) 和气相色谱结合质谱 (GC/MS) 分析来鉴定斑潜蝇的性信息素。通过 GC-EAD 分析，在女性性信息素腺提取物中检测到 3 个候选信息素，并通过对天然信息素成分和二甲基二硫加合物的质谱分析，将两种候选信息素鉴定为 (Z)-7-十二烯基乙酸酯 (Z7-12:OAc) 和 (Z)-9-十四碳烯基乙酸酯 (Z9-14:OAc)，比例为 1:5。在田间雄性诱捕试验中，使用二元混合物作为诱饵的诱捕器捕获了大量雄性，而使用单一成分的诱捕器几乎无法捕获雄性，这表明这两种成分对于雄性吸引力至关重要。此外，Z7-12:OAc 和 Z9-14:OAc 的最佳比例确定为 3:7–7:3，二元混合物的最佳剂量（Z7-12:OAc 和 Z9-14:OAc 之间的比例为 3:7）为 0.25–0.5 mg/陷阱，基于雄性渔获量。鉴定出一种极具吸引力的性信息素将有助于制定有效的策略来监测和控制飞虱。
Athetis lepigone has been recorded in many countries in Europe and Asia, but it had never been documented as an agricultural pest until 2005. For the purpose of using the sex pheromone to control this pest, we conducted a study to identify the sex pheromone of A. lepigone by gas chromatography with an electroantennographic detector (GC‐EAD) and GC coupled with mass spectrometry (GC/MS) analyses. Three pheromone candidates were detected by GC‐EAD analysis in the extracts of the female sex pheromone gland, and two candidates were identified as (Z)‐7‐dodecenyl acetate (Z7‐12:OAc) and (Z)‐9‐tetradecenyl acetate (Z9‐14:OAc) in a ratio of 1:5 by mass spectral analysis of natural pheromone components and dimethyl disulphide adducts. In the field male trapping test, the traps baited with the binary blend captured high number of males, while traps with single component hardly caught males, indicating that the two components are essential for the male attractiveness. In addition, the optimum ratios of Z7‐12:OAc and Z9‐14:OAc were determined as 3:7–7:3, and the best doses for the binary blend (at ratio of 3:7 between Z7‐12:OAc and Z9‐14:OAc) were 0.25–0.5 mg/trap, based on the number of male catches. The identification of a highly attractive sex pheromone will help in developing efficient strategies for monitoring and control of A. lepigone.