Role of the Median Neurosecretory Cells in the Ovarian Development of the Blow Fly Protophormia terraenovae
Role of the Median Neurosecretory Cells in the Ovarian Development of the Blow Fly Protophormia terraenovae
复制标题
中位神经分泌细胞在苍蝇 Protophormia terraenovae 卵巢发育中的作用
DOI：
10.2108/zsj.16.187
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Shiga
中科院分区：
文献类型：
--
作者：
I. Toyoda;H. Numata;S. Shiga
摘要用二硫堇-二硫堇-品红染色法标记了地丽蝇滞育和非滞育成虫大脑各半球的10-14个正中神经分泌细胞（MNC）。对成年雌性的脑-后脑复合体进行了各种外科手术，以研究这些细胞在卵巢发育和滞育中的作用。在LD 18：6（18 h光照和6 h黑暗）和25°C的滞育避免条件下，大多数完整和假手术雌性具有卵黄发生卵巢，而所有MNC完全去除的雌性具有未成熟的卵巢。去除MNC的雌性动物的咽侧体（CA）显著小于完整和假手术组。切断心体神经后，大多数雌性卵巢未成熟。即使在切断心脏返神经后，仍有约60%的雌性存在卵黄发生卵巢。然而，在去除心脏体和下脑神经节复合体（CCHG）后，没有雌性动物具有卵黄发生卵巢。在LD 12：12和20°C的滞育诱导条件下，大多数雌性具有未成熟的卵巢，并且无论手术程序如何，CA都很小。结果提示，MNC可能分泌一种促卵黄发生的异源因子。该因子似乎从CCHG和部分主动脉释放到血淋巴中。
Abstract Staining with paraldehyde-thionin-paraldehyde-fuchsin labeled 10–14 median neurosecretory cells (MNC) in each hemisphere of the brain of diapause and nondiapause adults of the blow fly, Protophormia terraenovae (Diptera: Calliphoridae). Various surgical operations were performed on the brain-retrocerebral complex of female adults to investigate the role of these cells in ovarian development and diapause. Under the diapause-averting conditions of LD 18:6 (18 h light and 6 h darkness) and 25°C, most of the intact and sham-operated females had vitellogenic ovaries, whereas all of the females with the MNC completely removed had immature ovaries. The corpus allatum (CA) was significantly smaller in the MNC-removed females than in the intact and sham-operated ones. After severance of the nervi corporis cardiaci, most of the females had immature ovaries. Even after severance of the cardiac recurrent nerve, there were vitellogenic ovaries in about 60 % of the females. However, after removal of the corpus cardiacum and hypocerebral ganglion complex (CCHG), no females had vitellogenic ovaries. Under the diapause-inducing conditions of LD 12:12 and 20°C, most females had immature ovaries and the CA was small irrespective of the surgical procedures. The results indicate that the MNC may secrete an allatotropic factor to stimulate vitellogenesis. The factor seems to be released to the hemolymph from the CCHG and a part of the aorta.