USE OF PROPIDIUM IODIDE STAINING AND FLOW-CYTOMETRY TO MEASURE ANTIBODY-MEDIATED CYTO-TOXICITY - RESOLUTION OF COMPLEMENT-SENSITIVE AND RESISTANT TARGET-CELLS
USE OF PROPIDIUM IODIDE STAINING AND FLOW-CYTOMETRY TO MEASURE ANTIBODY-MEDIATED CYTO-TOXICITY - RESOLUTION OF COMPLEMENT-SENSITIVE AND RESISTANT TARGET-CELLS
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DOI：
10.1016/0022-1759(83)90115-1
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
PIPHO, C
中科院分区：
文献类型：
--
作者：
JACOBS, DB;PIPHO, C
使用碘化丙啶摄取作为细胞活力的指标来测量抗体介导的细胞毒性。与使用荧光激活细胞分选仪的分析相结合，该方法与同位素释放测定系统一样灵敏，并且具有收集单个细胞的多参数数据的优势。该特征使得能够将抗体结合靶[鼠]细胞分解为对补体介导的杀伤敏感的群体和对补体介导的杀伤具有抗性的另一群体。
Propidium iodide uptake as an indicator of cell viability was used to measure antibody-mediated cytotoxicity. In conjunction with analysis using the fluorescence-activated cell sorter, this method is as sensitive as isotope release assay systems and has the advantage of gathering multiparameter data on individual cells. This feature enables the resolution of antibody-binding target [murine] cells into a population which is sensitive to complement-mediated killing and another population which is resistant to complement-mediated killing.