Dynamic patterned expression of orphan nuclear receptor genes RORalpha and RORbeta in developing mouse forebrain.
Dynamic patterned expression of orphan nuclear receptor genes RORalpha and RORbeta in developing mouse forebrain.
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发表时间：
2003
影响因子：
2.9
通讯作者：
Y. Nakagawa;D. O'Leary
中科院分区：
文献类型：
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作者：
Y. Nakagawa;D. O'Leary
作为使用两个密切相关的成员的核受体家族，RORalpha和RORbeta，作为标记物和工具，在小鼠前脑的遗传操作的一步，我们已经使用原位杂交分析其表达从E10.5到P7。在胚胎后期和出生后早期，ROR α在背侧丘脑的表达主要局限于整个主要感觉核团的强烈表达。ROR β在与ROR α类似的丘脑背核中表达，但在主要感觉核中表现出更有限的表达。ROR α早在E12.5在背侧丘脑中由假定的腹后神经元表达，而ROR β表达直到胚胎后期才被检测到。ROR β在胚胎新皮质中高度表达，并表现出强烈的分级的喙尾和侧内侧表达模式。在出生后的第一周，ROR β的分级表达逐渐获得分离模式，主要限于初级感觉区的第4层和第5层。相反，非常弱的ROR α表达首先在出生前后的新皮质中检测到，并且仅限于假定的躯体感觉区的皮质板的中间层。后来，一个有限的和非常弱的ROR α表达是明显的，主要是在第4层的尾部地区。为了确定ROR α和ROR β在出生后的正确表达和模式化是否需要模式化的视网膜输入，我们进行了新生儿双侧眼球摘除术。我们没有检测到任何显着差异，正常和去核小鼠在视觉领域的表达。虽然TCA输入可能需要适当的调节出生后的RORalpha和RORbeta的表达，这些研究结果表明，这些基因的动态出生后的表达和图案化的方面进行调节独立于图案化的视觉活动由膝状皮质传入中继。ROR α在背侧丘脑中的模式化表达表明，该基因位点可能有助于遗传修饰背侧丘脑和丘脑皮质投射的发育。
As a step toward using two closely related members of the nuclear receptor family, RORalpha and RORbeta, as markers and tools for genetic manipulations in mouse forebrain, we have used in situ hybridization to analyze their expression from E10.5 to P7. At later embryonic and early postnatal ages, RORalpha expression in dorsal thalamus is mainly limited to robust expression throughout the principal sensory nuclei. RORbeta is expressed in a similar set of dorsal thalamic nuclei as RORalpha, but exhibits a more limited expression within the principal sensory nuclei. RORalpha is expressed as early as E12.5 in dorsal thalamus by presumptive ventroposterior neurons, whereas RORbeta expression is not detected until later embryonic ages. RORbeta is highly expressed in embryonic neocortex, and exhibits strongly graded rostrocaudal and lateromedial patterns of expression. Over the first postnatal week, the graded expression of RORbeta gradually acquires a disjunctive pattern largely restricted to layers 4 and 5 of the primary sensory areas. In contrast, very weak RORalpha expression is first detected in the neocortex just around birth, and is limited to the middle layer of the cortical plate of the putative somatosensory area. Later, a limited and very weak RORalpha expression is evident mainly in layer 4 of more caudal areas. To determine whether patterned retinal input is required for the proper postnatal expression and patterning of RORalpha and RORbeta, we performed neonatal bilateral enucleations. We did not detect any significant differences between normal and enucleated mice in expression in visual areas. Although TCA input may be required for proper regulation of the postnatal expression of RORalpha and RORbeta, these findings suggest that aspects of the dynamic postnatal expression and patterning of these genes are regulated independently of patterned visual activity relayed by geniculocortical afferents. The patterned expression of RORalpha in dorsal thalamus suggests that this gene locus may be useful to genetically modify the development of dorsal thalamus and thalamocortical projections.