A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro
A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro
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DOI：
10.1016/s0092-8674(00)81785-7
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发表时间：
1998-10-02
期刊：
影响因子：
64.5
通讯作者：
Emerson, BM
中科院分区：
文献类型：
--
作者：
Armstrong, JA;Bieker, JJ;Emerson, BM
红系Kruppel样因子（EKLF）是人类β-珠蛋白基因阶段特异性表达所必需的。我们发现，EKLF需要一个SWI/SNF相关的染色质重塑复合物，EKLF共激活因子重塑复合物1（E-RC 1），在体外染色质模板上产生一个DNase I超敏感的，转录活性的β-珠蛋白启动子。E-RC 1包含酵母SWI/SNF亚基的BRG 1、BAF 170、BAF 155和INI 1（BAF 47）同源物，以及高等真核生物特有的亚基BAF 57，其对染色质重塑和EKLF转录至关重要。E-RC 1显示对转录因子的功能选择性，因为它不能用E盒结合蛋白TFE-3激活染色质组装的HIV-1模板的表达。因此，SWI/SNF家族的成员直接参与转录激活，并可通过选择性地与特异性DNA结合蛋白相互作用来调节基因亚群。
Erythroid Kruppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 (BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.