SlJMJ7 orchestrates tomato fruit ripening via crosstalk between H3K4me3 and DML2-mediated DNA demethylation
SlJMJ7 orchestrates tomato fruit ripening via crosstalk between H3K4me3 and DML2-mediated DNA demethylation
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SlJMJ7 通过 H3K4me3 和 DML2 介导的 DNA 去甲基化之间的串扰协调番茄果实成熟
DOI：
10.1111/nph.17838
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发表时间：
2022-02-01
期刊：
影响因子：
9.4
通讯作者：
Duan, Xuewu
中科院分区：
文献类型：
--
作者：
Ding, Xiaochun;Liu, Xuncheng;Duan, Xuewu
肉质果实的成熟是一个独特的发育过程，拟南芥和水稻缺乏。这个过程是由激素和转录因子驱动的。然而，对果实成熟的关键和早期调节因子仍然知之甚少。在这里，我们揭示了SlJMJ7，一个H3K4脱甲基酶，是番茄果实成熟的关键负调节因子。结合全基因组转录、结合位点、组蛋白H3K4me3和DNA甲基化分析表明，SlJMJ7通过H3K4me3去甲基化调控一组关键的成熟相关基因，包括乙烯生物合成（ACS2、ACS4和ACO6）、转录调控（RIN和NOR）和DNA去甲基化（DML2）基因。此外，SlJMJ7功能的丧失导致H3K4me3水平增加，其直接激活成熟相关基因，并导致果实中DML2介导的DNA低甲基化，其间接促进成熟相关基因的表达。总之，这些效应导致sljmj7突变体的果实加速成熟。我们的研究结果表明，SlJMJ7作为一个主负调节水果成熟不仅通过直接去除H3K4me3从多个关键成熟相关的因素，但也通过组蛋白和DNA去甲基化之间的串扰。这些发现揭示了组蛋白甲基化和DNA甲基化之间的一种新的串扰，以调节植物发育过程中的基因表达。
The ripening of fleshy fruits is a unique developmental process that Arabidopsis and rice lack. This process is driven by hormones and transcription factors. However, the critical and early regulators of fruit ripening are still poorly understood. Here, we revealed that SlJMJ7, an H3K4 demethylase, is a critical negative regulator of fruit ripening in tomato. Combined genome-wide transcription, binding sites, histone H3K4me3 and DNA methylation analyses demonstrated that SlJMJ7 regulates a key group of ripening-related genes, including ethylene biosynthesis (ACS2, ACS4 and ACO6), transcriptional regulation (RIN and NOR) and DNA demethylation (DML2) genes, by H3K4me3 demethylation. Moreover, loss of SlJMJ7 function leads to increased H3K4me3 levels, which directly activates ripening-related genes, and to global DML2-mediated DNA hypomethylation in fruit, which indirectly prompts expression of ripening-related genes. Together, these effects lead to accelerated fruit ripening in sljmj7 mutant. Our findings demonstrate that SlJMJ7 acts as a master negative regulator of fruit ripening not only through direct removal of H3K4me3 from multiple key ripening-related factors, but also through crosstalk between histone and DNA demethylation. These findings reveal a novel crosstalk between histone methylation and DNA methylation to regulate gene expression in plant developmental processes.