Lampropedia aestuarii sp. nov., isolated from the estuary of a freshwater river
Lampropedia aestuarii sp. nov., isolated from the estuary of a freshwater river
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河口灯足属 sp.
DOI：
10.1099/ijsem.0.003710
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发表时间：
2020
影响因子：
2.8
通讯作者：
Yong-Xia Wang
中科院分区：
文献类型：
--
作者：
Yi-Ting Zhao;Ya-Ping Li;Wei Xiao;Wei-Hong Liu;Xiao-Long Cui;Yong-Xia Wang
从马料河入海口沉积物中分离到一株革兰氏阴性、严格好氧、过氧化氢酶阳性和氧化酶阳性的细菌，命名为YIM MLB 12 T。细胞呈非运动性杆状。在15-35 °C（最佳，25-30 °C）、pH 6.0-10.0（最佳，pH 7.0-8.0）和0- 7%（w/v）NaCl（最佳，0.5- 2%）存在下观察到生长。 基于16 S rRNA基因序列的系统发育分析结果表明，菌株YIM MLB 12 T与Lampropedia属成员形成了紧密的系统发育谱系，与“Lampropedia puyangensis”2-bin关系密切，序列相似性为98.3%，与Lampropedia hyalina ATCC 11041 T（96%）和Lampropedia cohaerens CT 6 T（95.5%）模式菌株的相似性较低。 YIM MLB 12 T与L. puyangensis的KCTC 32235分别为76.5%和22.6%。 菌株YIM MLB 12 T含有主要醌类化合物泛醌-8。主要的细胞脂肪酸是合计特征3（C16：1ω 7 c和/或C16：1ω6c）、C16：0、C10：03-OH、合计特征8（C18：1ω 6c和/或C18：1ω 7 c）、C12：03-OH和C14：0。 YIM MLB 12 T的极性脂主要由二磷脂酰甘油、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺和磷脂酰甘油组成。主要的多胺是亚精胺。菌株YIM MLB 12 T基因组DNA G+C含量为56.8mol%。根据其基因型和化学分类学特征以及表型分析结果，菌株YIM MLB 12 T代表一个新种，命名为Lampropedia aestuariisp。11月。模式菌株为YIM MLB 12 T（=KCTC 42886 T =CGMCC 1.17071T）。
A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain YIM MLB12T, was isolated from estuary sediment sampled at Maliao River where it flows into a plateau lake (Dianchi) in Yunnan, south-west PR China. Cells were non-motile and rod-shaped. Growth was observed at 15–35 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7 % (w/v) NaCl (optimum, 0.5–2 %). Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM MLB12Tformed a tight phylogenic lineage with members of the genusLampropediaand was closely related to ‘Lampropedia puyangensis’ 2-bin with 98.3 % sequence similarity and had low similarities to the type strains ofLampropediahyalinaATCC 11041T(96 %) andLampropedia cohaerensCT6T(95.5 %). Average nucleotide identity andin silicoDNA–DNA hybridization values between strain YIM MLB12Tand ‘L. puyangensis’ KCTC 32235 were 76.5 and 22.6 %, respectively. Strain YIM MLB12Tcontained ubiquinone-8 as the major quinone. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c), C16 : 0, C10 : 03-OH, summed feature 8 (C18 : 1ω6cand/or C18 : 1ω7c), C12 : 03-OH and C14 : 0. The polar lipid profile of strain YIM MLB12Twas composed predominantly of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The major polyamine was spermidine. The genomic DNA G+C content of strain YIM MLB12Twas 56.8 mol%. Based on its genotypic and chemotaxonomic features and results of phenotypic analyses, strain YIM MLB12Trepresents a novel species of the genusLampropedia, for which the nameLampropedia aestuariisp. nov. is proposed. The type strain is YIM MLB12T(=KCTC 42886T=CGMCC 1.17071T).