Derma-like antibacterial polysaccharide gel dressings for wound care
Derma-like antibacterial polysaccharide gel dressings for wound care
复制标题
用于伤口护理的类真皮抗菌多糖凝胶敷料
DOI：
10.1016/j.actbio.2022.06.018
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发表时间：
2022
影响因子：
9.7
通讯作者：
Fu-Jian Xu
中科院分区：
文献类型：
--
作者：
Yan-Hua Xiong;Lujiao Zhang;Zongpeng Xiu;Bingran Yu;Shun Duan;Fu-Jian Xu
大面积皮肤伤口感染发病率高，严重威胁人类健康。因此，研制能阻断微生物侵入、有效杀灭细菌、牢固粘附于创面、生物相容性好的新型创面敷料是十分必要的。本研究设计了一种具有良好创面护理性能的类真皮结构多糖凝胶敷料。利用氧化透明质酸溶液与高粘度羧甲基壳聚糖溶液的Schiff碱反应，通过渗透交联的方法，在DLG内部形成模拟真皮结构的梯度多孔结构，这是由于两种粘性溶液同时渗透和反应的结果。这种类真皮结构赋予了凝胶敷料对伤口的自粘附能力和对细菌的屏障能力。通过引入乌贼汁和庆大霉素，在808 nm近红外线照射下，改性凝胶敷料（DLG-GS）显示出温和的光热效应，可达到并维持45 °C的温度。温和的加热能与庆大霉素共同作用，在5 min内产生快速杀菌性能，同时多糖凝胶敷料具有良好的生物相容性。通过感染性全层皮肤缺损的动物模型证明了DLG-GS的体内抗感染特性。该策略为大面积创面感染的防治提供了一种可行的解决方案。重要性声明：采用渗透交联法制备了一种具有高细菌屏障能力、强组织粘附性和良好生物相容性的类真皮抗菌凝胶敷料（DLG GS）。DLG-GS由于其温和的光热效应和抗生素的合理组合，可在5 min内消除细菌感染。在感染性全层皮肤缺损的动物模型中，DLG-GS显示出高的抗感染和伤口愈合特性。本研究为抗菌伤口敷料的开发提供了一种灵活通用的策略。
Large skin wound infections have high morbidity, which threaten the health of human beings severely. It is essential to develop new wound dressings that can block microbial invasion, eliminate bacteria effectively, adhere to wounds firmly, and have good biocompatibility. In this work, we designed a kind of polysaccharide gel (DLG) dressings with derma-like structure that had good wound care performances. With a facile penetration cross-linking method by the Schiff base reaction between oxidized hyaluronic acid solution and carboxymethyl chitosan solution with higher viscosity, a gradient porous structure was formed inside DLG to mimic the structure of derma, which was due to the simultaneous penetration and reaction processes between two viscous solutions. This derma-like structure endowed the gel dressings with the abilities of self-adhesion to wounds and barriers against bacteria. Through the introduction of cuttlefish juice and gentamycin, the modified gel dressings (DLG-GS) showed mild photothermal effects under the near infrared irradiation at the wavelength of 808 nm, which could reach and maintain the temperature of 45 °C. The mild heat could act together with gentamycin to produce a rapid bactericidal performance within 5 min. Meanwhile, the polysaccharide gel dressings had good biocompatibility. The in vivo anti-infection properties of DLG-GS was demonstrated by an animal model of infected full-thickness skin defect. This strategy provided a feasible solution for the prevention and treatment of infected large wounds. STATEMENT OF SIGNIFICANCE: Derma-like antibacterial gel dressings (DLG-GS) with high bacterial barrier ability, strong tissue adhesive property and good biocompatibility were constructed by a penetration cross-linking method. DLG-GS could eliminate bacterial infection within 5 min due to the rational combination of a mild photothermal effect and antibiotics. DLG-GS showed high anti-infection and wound healing properties in an animal model of infected full-thickness skin defect. This study provides a flexible and universal strategy for the development of antibacterial wound dressings.