Effect of rat-to-mouse bioartificial pancreas xenotransplantation on diabetic renal damage and survival
Effect of rat-to-mouse bioartificial pancreas xenotransplantation on diabetic renal damage and survival
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DOI：
10.1097/01.mpa.0000203959.31877.8c
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发表时间：
2006-04-01
期刊：
影响因子：
2.9
通讯作者：
Inoue, Kazutomo
中科院分区：
文献类型：
--
作者：
Sakata, Naoaki;Gu, Yuanjun;Inoue, Kazutomo
目的：糖尿病肾病是威胁糖尿病患者生命的并发症。生物人工胰腺移植正在成为治疗糖尿病的一种选择，因为它可以保护同种和异种胰岛免受宿主免疫系统的攻击。本研究旨在探讨生物人工胰腺移植在改善或预防糖尿病肾损害中的作用。方法：将约800只大鼠胰岛用聚乙烯醇凝胶包裹，然后移植到糖尿病小鼠的腹膜内(移植组)。移植无胰岛胶囊的糖尿病小鼠为假手术组。结果：与假手术组比较，TX组大鼠存活时间、血糖、血尿素氮、血肌酐、尿常规、尿水量、肾组织学改变均有明显改善。两组患者尿液参数无明显变化，饮水量也无明显差异。组织学检查显示TX组大鼠肾小球系膜基质扩张减少。结论：本研究证实了聚乙烯醇凝胶生物人工胰腺移植对糖尿病肾脏的保护作用。
Objective: Diabetic nephropathy is a life-threatening complication of diabetes mellitus. Bioartificial pancreas transplantation is becoming a therapeutic option for diabetes mellitus as it protects both allogeneic and xenogeneic islets from the host immune system. This study was undertaken to determine the effectiveness of bioartificial pancreas transplantation to improve or prevent diabetic renal damage.Methods: Approximately 800 rat islets were macroencapsulated in polyvinyl alcohol gel and then transplanted into the peritoneal cavity of diabetic mice (transplantation group [Tx group]). Diabetic mice transplanted with a capsule without islets served as a sham operation group. After transplantation, the following data were collected: survival, body weight, blood glucose, blood urea nitrogen, serum creatinine levels, urinalysis, water intake, and histological changes in the kidney.Results: There was a significant improvement in survival, blood glucose, blood urea nitrogen, and creatinine in the Tx group compared with the sham operation group. No remarkable changes were seen in urinary parameters between the 2 groups, and there was also no significant difference in water intake. Histological examination revealed that mesangial matrix expansion was decreased in the Tx group.Conclusions: This study demonstrated that polyvinyl alcohol gel bioartificial pancreas transplantation can protect the kidney from diabetic damage.