Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses
Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses
复制标题
DOI：
10.1002/eji.1830270935
复制
发表时间：
1997-09-01
影响因子：
5.4
通讯作者：
Kearney, JF
中科院分区：
文献类型：
--
作者：
Oliver, AM;Martin, F;Kearney, JF
小鼠脾B细胞可根据其独特的细胞表面抗原的表达进行分离，边缘带(MZ)B细胞为CD38(高)CD21(高)CD23(低/-)，滤泡(FO)B细胞为CD21(INT)CD23(INT)，新形成的(NF)B细胞为CD21(DUN/-)CD23(-)。利用这些表型差异，我们分离了三个B细胞亚群，并在体外评估了它们的其他表型差异和功能。在IgM或CD38交联剂作用下，FO B细胞的增殖能力高于其他B细胞亚群。在脂多糖(LPS)刺激下，MZ B细胞的增殖能力强于FO B细胞，而LPS和CD38交联剂或CD40结扎剂的刺激效果较差。当NE-FO和MZ-B细胞被内毒素或内毒素和白介素4刺激时，MZ-B细胞比其他两个亚群分泌更多的IgM和IgG3，同样，B细胞受体交联后，MZ-B细胞内测得的钙离子通量比其他两个亚群的更大且更持久。综上所述，这些结果表明CB38(高)CD21(高)CD23(低/-)MZ B细胞特别适合在对递送到MZ区的抗原作出反应时发挥独特的作用。
Mouse splenic B cells can be separated based on their distinctive expression of cell surface antigens, Marginal zone (MZ) B cells are CD38(high) CD21(high) CD23(low/-), while follicular (FO) B cells are CD21(int) CD23(int) and newly formed (NF) B cells are CD21(dun/-) CD23(-). Exploiting these phenotypic distinctions, we isolated the three B cell subsets and assessed their Other phenotypic differences and functional capabilities in vitro. FO B cells proliferate more than the other B cell subsets in response to either IgM or CD38 cross-linking, MZ B cells proliferate better than FO B cells when stimulated with lipopolysaccharide (LPS), sub-optimal levels of LPS and CD38 cross-linking or CD40 ligation. When NE FO and MZ B cells were stimulated with either LPS or LPS and interleukin-4, MZ B cells secreted more IgM and IgG3 than the other two subsets, Similarly, calcium fluxes measured within MZ B cells are larger in amplitude and more sustained after B cell receptor cross-linking than those found in the other two subsets. Collectively, these results indicate that CB38(high) CD21(high) CD23(low/-) MZ B cells are specially suited to play a unique role in response to antigens delivered to the MZ area.