Trafficking of UL37 Proteins into Mitochondrion-Associated Membranes during Permissive Human Cytomegalovirus Infection
Trafficking of UL37 Proteins into Mitochondrion-Associated Membranes during Permissive Human Cytomegalovirus Infection
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DOI：
10.1128/jvi.00885-10
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发表时间：
2010-08-01
影响因子：
5.4
通讯作者：
Colberg-Poley, Anamaris M.
中科院分区：
文献类型：
--
作者：
Bozidis, Petros;Williamson, Chad D.;Colberg-Poley, Anamaris M.
人巨细胞病毒（HCMV）UL 37蛋白从内质网（ER）依次运输到线粒体。在瞬时转染的细胞中，UL 37蛋白进入内质网和线粒体之间的接触部位--线粒体相关膜（MAM）。在HCMV感染的细胞中，主要的UL 37外显子1蛋白pUL 37 x1被运输到ER、MAM和线粒体中。令人惊讶的是，MAM钙信号连接复合物的组分，胞质Grp 75，越来越多地富集在来自HCMV感染细胞的重MAM中。这些研究显示了第一例有记录的疱疹病毒蛋白（HCMV pUL 37 x1）在允许感染期间贩运到MAM以及HCMV诱导的MAM蛋白组成改变的病例。
Human cytomegalovirus (HCMV) UL37 proteins traffic sequentially from the endoplasmic reticulum (ER) to the mitochondria. In transiently transfected cells, UL37 proteins traffic into the mitochondrion-associated membranes (MAM), the site of contact between the ER and mitochondria. In HCMV-infected cells, the predominant UL37 exon 1 protein, pUL37x1, trafficked into the ER, the MAM, and the mitochondria. Surprisingly, a component of the MAM calcium signaling junction complex, cytosolic Grp75, was increasingly enriched in heavy MAM from HCMV-infected cells. These studies show the first documented case of a herpesvirus protein, HCMV pUL37x1, trafficking into the MAM during permissive infection and HCMV-induced alteration of the MAM protein composition.