INSULIN-DEPENDENT REGULATION OF INSULIN RECEPTOR CONCENTRATIONS - DIRECT DEMONSTRATION IN CELL-CULTURE
INSULIN-DEPENDENT REGULATION OF INSULIN RECEPTOR CONCENTRATIONS - DIRECT DEMONSTRATION IN CELL-CULTURE
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DOI：
10.1073/pnas.71.1.84
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
BUELL, DN
中科院分区：
文献类型：
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作者：
GAVIN, JR;ROTH, J;BUELL, DN
将培养的人淋巴细胞长期（5-16小时）暴露于37°10-8M的胰岛素中，会产生胰岛素受体浓度的降低，这是单纯占据位置所无法解释的；急性暴露（0-2小时）无影响。这些结果重复了在体内观察到的结果，其中慢性高胰岛素血症（例如，肥胖胰岛素抵抗高血糖小鼠循环中10-8M胰岛素）与每个细胞胰岛素受体浓度的显著降低有关，而急性高胰岛素血症对受体浓度没有影响。这些数据表明，细胞外液中的胰岛素与每个细胞的胰岛素受体浓度之间存在相互关系，这是通过细胞内胰岛素敏感调节过程在靶细胞本身介导的，并直接影响靶细胞对激素的敏感性。
Chronic (5-16 hr) exposure of cultured human lymphocytes to 10-8M insulin at 37°in vitroproduced a decrease in insulin receptor concentrations unaccounted for by simple occupancy of sites; acute exposure (0-2 hr) was without effect. These results reproduced observationsin vivowhere chronic hyperinsulinemia (e.g., 10-8M insulin in the circulation of obese insulinresistant hyperglycemic mice) is associated with a substantial reduction in the concentration of insulin receptors per cell, while acute hyperinsulinemiain vivohas no effect on receptor concentration. These data suggest a reciprocal relationship between insulin in the extracellular fluid and the concentration of insulin receptors per cell, which is mediated at the target cell itself by intracellular insulin-sensitive regulatory processes and directly affects target-cell sensitivity to hormone.