Thermal conductivity of a Jurkat cell measured by a transient laser point heating method
Thermal conductivity of a Jurkat cell measured by a transient laser point heating method
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DOI：
10.1016/j.ijheatmasstransfer.2020.120161
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发表时间：
2020-10
影响因子：
5.2
通讯作者：
R. Shrestha;R. Atluri;D. Simmons;Dongsik Kim;Tae-youl Choi
中科院分区：
文献类型：
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作者：
R. Shrestha;R. Atluri;D. Simmons;Dongsik Kim;Tae-youl Choi
为了理解和量化生物细胞中的热能传递，在细胞水平上测量热特性变得非常重要。我们在此报告一种独特的技术，利用激光点热源的时间温度上升的微量移液管热传感器，这种技术的特点是热传导的测量样品，Jurkat细胞，从而测量样品的热导率（TC）。为此，我们在COMSOL中结合计算模型来求解瞬态温度，并使用MATLAB对实验数据进行多参数拟合。为了解决Jurkat细胞的化学组成对TC的影响，我们比较了三种结构模型，用于预测异质材料中的有效热导率，从而确定Jurkat细胞的重量百分比。当考虑水和蛋白质作为主要成分时，我们发现Maxwell-Euken和有效介质理论模型的组合提供了与已公布的重量百分比数据最接近的近似值，因此，建议用于预测细胞组成。我们通过测量聚乙烯微球来验证测量技术本身的准确性，并观察到与已发表数据的1%偏差。该独特技术被确定为机械非侵入性的，能够维持活细胞，并且能够测量Jurkat细胞的热导率，其被证明为0.538 W/（m·K）± 1%。
To understand and quantify the thermal energy transfer in a biological cell, the measurement of thermal properties at a cellular level is emerging as great importance. We report herein a unique technique that utilizes a laser point heat source for temporal temperature rise in a micro-pipette thermal sensor; this technique characterizes heat conduction of a measured sample, the Jurkat cell, thus measuring the sample's thermal conductivity (TC). To this end, we incorporated the computational model in COMSOL to solve for the transient temperature and used the multi-parameter fitting of the experimental data using MATLAB. To address the influence of a Jurkat cell's chemical composition on TC, we compared three structural models for prediction of effective thermal conductivity in heterogeneous materials thereby determining the weight percentage of the Jurkat cell. When considering water and protein as the major constituents, we found that a combination of Maxwell-Euken and Effective Medium Theory modeling provides the closest approximation to published weight percent data and, therefore, is recommended for prediction of the cell composition. We validate the accuracy of the measurement technique, itself, by measuring polyethylene microspheres and observed 1% deviation from published data. The unique technique was determined to be mechanically non-invasive, capable of maintaining viable cells, and capable of measuring the thermal conductivity of a Jurkat cell, which was demonstrated to be 0.538 W/(m⋅K) ± 1%.