Inhibition of HIV-1 replication by a Tat transdominant negative mutant in human peripheral blood lymphocytes from healthy donors and HIV-1-infected patients
Inhibition of HIV-1 replication by a Tat transdominant negative mutant in human peripheral blood lymphocytes from healthy donors and HIV-1-infected patients
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Tat 转显性阴性突变体对健康捐献者和 HIV-1 感染患者外周血淋巴细胞中 HIV-1 复制的抑制
作者：
C. Rossi;P. Balboni;M. Betti;P. Marconi;R. Bozzini;M. Grossi;G. Barbanti‐Brodano;A. Caputo
先前表明，其中半胱氨酸22被甘氨酸取代的tat突变体（tat 22）在被HIV-1裂解或潜伏感染的Jurkat T细胞中表现为反式显性负突变体。在这项研究中，我们证明了tat 22控制HIV-1在原代细胞中的复制。在体外感染来自正常供体的外周血单核细胞（PBMC）和血清阳性患者的PBMC中的潜伏感染重新激活后，均观察到这种效应。tat 22的抗病毒作用仅限于低病毒产生的条件。使用tat 22可能是有希望的基因治疗方法，艾滋病在无症状阶段的疾病，允许控制病毒复制感染的细胞和抑制病毒传播到未感染的细胞。
It was previously shown that a tat mutant (tat22) where cysteine 22 is substituted by glycine behaves as a transdominant negative mutant in Jurkat T cells lytically or latently infected by HIV-1. In this study we demonstrate that tat22 controls HIV-1 replication in primary cells. This effect was observed both after in vitro infection of peripheral blood mononuclear cells (PBMCs) from normal donors and after reactivation of the latent infection in PBMCs from seropositive patients. The antiviral effect of tat22 was limited to conditions of low virus production. The use of tat22 may be promising for a gene therapy approach to AIDS during the asymptomatic phase of the disease allowing control of virus replication in infected cells and inhibition of virus spread to uninfected cells.
DOI：
10.1073/pnas.90.16.7632
发表时间：
1993-08-15
影响因子：
11.1
作者：
FLORES, SC;MARECKI, JC;MCCORD, JM
通讯作者：
MCCORD, JM
保护性基因的表达可延长人类免疫缺陷病毒感染患者 T 细胞的存活时间。
DOI：
10.1073/pnas.93.7.2889
发表时间：
1996
影响因子：
11.1
作者：
Woffendin,C;Ranga,U;Yang,Z;Xu,L;Nabel,GJ
通讯作者：
Nabel,GJ
影响因子：
3.7
作者：
Kamine, J;Elangovan, B;Chinnadurai, G
通讯作者：
Chinnadurai, G