Chloral hydrate alters the organization of the ciliary basal apparatus and cell organelles in sea urchin embryos
Chloral hydrate alters the organization of the ciliary basal apparatus and cell organelles in sea urchin embryos
复制标题
DOI：
10.1007/s004410051137
复制
发表时间：
1998-09-01
影响因子：
3.6
通讯作者：
Taylor, M
中科院分区：
文献类型：
--
作者：
Chakrabarti, A;Schatten, H;Taylor, M
有丝分裂抑制剂水合氯醛可诱导红尾绿绒螯蟹胚胎早期纤毛脱落。它会导致纤毛和称为基板的基体连接处的纤毛断裂。这使得质膜暂时打开。由基体、基足、基足帽、横纹侧臂和横纹小根组成的基底器附属结构通过水合氯醛处理而错位或解体。此外，与基底器相关的微管被分解。线粒体聚集在纤毛的基部-质膜下-并显示其结构组织的改变，在所有电子显微镜切片的40%中观察到线粒体的积累，而60%显示大多没有线粒体的区域。在水合氯醛存在下，纤毛和横纹根周围的微绒毛保持完整。这些结果表明，decilation在早期海胆胚胎水合氯醛是由纤毛膜和纤毛中的微管的联合作用。此外，有人建议，水合氯醛可以作为一种工具，探索参与纤毛运动的海胆胚胎发育中的细胞骨架机制。
The mitotic inhibitor, chloral hydrate, induces ciliary loss in the early embryo phase of Lytechinus pictus. It causes a breakdown of cilia at the junction of the cilium and the basal body known as the basal plate. This leaves the plasma membrane temporarily unsealed. The basal apparatus accessory structures, consisting of the basal body, basal foot, basal foot cap, striated side arm, and striated rootlet, are either misaligned or disintegrated by treatment with chloral hydrate. Furthermore, microtubules which are associated with the basal apparatus are disassembled. Mitochondria accumulate at the base of cilia - underneath the plasma membrane - and show alterations in their structural organization, The accumulation of mitochondria is observed in 40% of all electron micrograph sections while 60% show the areas mostly devoid of mitochondria. The microvilli surrounding a cilium and striated rootlet remain intact in the presence of chloral hydrate. These results suggest that deciliation in early sea urchin embryos by chloral hydrate is caused by combined effects on the ciliary membrane and on microtubules in the cilia. Furthermore, it is suggested that chloral hydrate can serve as a tool to explore the cytoskeletal mechanisms that are involved in cilia motility in the developing sea urchin embryo.