Dual functions of phospholipase Dα1 in plant response to drought
Dual functions of phospholipase Dα1 in plant response to drought
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DOI：
10.1093/mp/ssm025
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发表时间：
2008-03-01
期刊：
影响因子：
27.5
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Hong, Yueyun;Zheng, Suqin;Wang, Xuemin
磷脂酶D α 1（PLD α 1）已被证明介导脱落酸调节气孔运动。拟南芥植物缺乏PLD α 1增加，而PLD α 1过表达烟草减少，蒸腾失水。在干旱的早期阶段，水分损失的减少与PLD α 1过表达植物中PLD的高水平引起的气孔快速关闭有关。但在干旱后期，过表达植株表现出比对照植株更强的干旱敏感性。PLD α 1活性在过表达的植物是远远高于对照植物，其中干旱也诱导PLD α 1活性的增加。高水平的PLD α 1活性与干旱后期的膜降解有关，如离子渗漏和脂质过氧化所示。这些结果表明，PLD α 1的高水平表达对植物响应水分亏缺有不同的影响。它促进气孔关闭在早期阶段，但在长期干旱胁迫破坏膜。这些研究结果进行了讨论PLD功能和潜在的应用的理解。
Phospholipase D alpha 1 (PLD alpha 1) has been shown to mediate the abscisic acid regulation of stomatal movements. Arabidopsis plants deficient in PLD alpha 1 increased, whereas PLD alpha 1-overexpressing tobacco decreased, transpirational water loss. In the early stage of drought, the decrease in water loss was associated with a rapid stomatal closure caused by a high level of PLD in PLD alpha 1-overexpressing plants. However, in the late stage of drought, the overexpressing plants displayed more susceptibility to drought than control plants. PLD alpha 1 activity in the overexpressing plants was much higher than that of control plants in which drought also induced an increase in PLD alpha 1 activity. The high level of PLD alpha 1 activity was correlated to membrane degradation in late stages of drought, as demonstrated by ionic leakage and lipid peroxidation. These findings indicate that a high level of PLD alpha 1 expression has different effects on plant response to water deficits. It promotes stomatal closure at earlier stages, but disrupts membranes in prolonged drought stress. These findings are discussed in relation to the understanding of PLD functions and potential applications.