Lipidome and proteome map of myelin membranes
Lipidome and proteome map of myelin membranes
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DOI：
10.1002/jnr.23157
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发表时间：
2013-03-01
影响因子：
4.2
通讯作者：
Dhaunchak, Ajit S.
中科院分区：
文献类型：
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作者：
Gopalakrishnan, Gopakumar;Awasthi, Anshul;Dhaunchak, Ajit S.
为了了解髓磷脂膜的分子解剖结构，我们对新鲜纯化的人和鼠髓磷脂组分进行了大规模、基于液相色谱-串联质谱 (LC-MS/MS) 的脂质组和蛋白质组筛选。我们在这两个物种中鉴定了 700 多个脂质部分和 1,000 多个蛋白质，其中包括 284 种常见脂质和 257 种常见蛋白质。这项研究建立了人类和小鼠髓磷脂膜成分的第一个综合图谱。尽管这项研究表明人类和鼠类髓鞘质之间有许多相似之处，但每个物种中都有几种独特的成分被鉴定出来。未来关注种间差异的定量验证研究将验证髓磷脂膜的解剖结构。尽管如此，此处提出的组合脂质组和蛋白质组图谱仍可用作髓磷脂健康和疾病的参考库。 (c) 2012 年 Wiley 期刊公司。
To understand the molecular anatomy of myelin membranes, we performed a large-scale, liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS)-based lipidome and proteome screen on freshly purified human and murine myelin fractions. We identified more than 700 lipid moieties and above 1,000 proteins in the two species, including 284 common lipids and 257 common proteins. This study establishes the first comprehensive map of myelin membrane components in human and mice. Although this study demonstrates many similarities between human and murine myelin, several components have been identified exclusively in each species. Future quantitative validation studies focused on interspecies differences will authenticate the myelin membrane anatomy. The combined lipidome and proteome map presented here can nevertheless be used as a reference library for myelin health and disease. (c) 2012 Wiley Periodicals, Inc.