MORPHOGENESIS AND EXPERIMENTAL ASPECTS OF GROWTH AND DEVELOPMENT OF THE MALE GAMETOPHYTE OF GINKGO-BILOBA INVITRO
MORPHOGENESIS AND EXPERIMENTAL ASPECTS OF GROWTH AND DEVELOPMENT OF THE MALE GAMETOPHYTE OF GINKGO-BILOBA INVITRO
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DOI：
10.2307/2443964
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发表时间：
1987-12-01
影响因子：
3
通讯作者：
FRIEDMAN, WE
中科院分区：
文献类型：
--
作者：
FRIEDMAN, WE
对种子植物银杏的形态复杂的雄配子体的形态发生进行了体外研究。这些研究表明，尽管体外发育比体内的发育变化更大，但其特征仍然是三种不同生长模式的连续表达：管细胞的初始扩散生长、尖端生长的起始和建立（可能伴随分枝）以及配子体的径向肿胀。延时摄影显示，雄配子体的分支是由两种不同类型的起始事件引起的：一种是顶端（“初级分支”），另一种是亚顶端（“次级分支”）。生长培养基的实验操作表明，外源蔗糖水平强烈影响正常形态发生过程表达的可能性。高浓度的蔗糖往往会抑制最初扩散生长后尖端生长的开始以及配子体的分枝。低蔗糖水平似乎促进了配子体早期建立尖端生长和随后分枝的正常模式，类似于体内配子体的发育。
Morphogenesis in the morphologically complex male gametophyte of the seed plant Ginkgo biloba was investigated in vitro. These studies reveal that although development in vitro is far more variable than in vivo, it is still characterized by the sequential expression of three distinct patterns of growth: initial diffuse growth of the tube cell, initiation and establishment of tip growth (which may be accompanied by branching), and radial swelling of the gametophyte. Time lapse photography shows that branching of the male gametophyte results from two distinct types of initiation events: one apical ("primary branching"), and the other subapical ("secondary branching"). Experimental manipulation of the growth medium indicates that exogenous sucrose levels strongly influence the likelihood of expression of normal morphogenetic processes. High levels of sucrose tend to inhibit both the initiation of tip growth following initial diffuse growth, as well as branching of the gametophyte. Low sucrose levels appear to promote a normal pattern of early establishment of tip growth and subsequent branching of the gametophyte, similar to development of the gametophyte in vivo.