Pattern of formylmethionyl-leucyl-phenylalanine-induced luminol- and lucigenin-dependent chemiluminescence in human neutrophils
Pattern of formylmethionyl-leucyl-phenylalanine-induced luminol- and lucigenin-dependent chemiluminescence in human neutrophils
复制标题
人中性粒细胞中甲酰甲硫氨酰-亮氨酰-苯丙氨酸诱导的鲁米诺和光泽精依赖性化学发光的模式
作者：
C. Dahlgren;H. Aniansson;K. Magnusson
甲硫氨酰-亮氨酰-苯丙氨酸对中性粒细胞的刺激导致双峰鲁米诺依赖的化学发光模式。然而，当我们使用光敏素作为放大物质时，我们只观察到在对多肽的反应中只有一个峰的化学发光模式。我们认为，较大的分子光亮素(510道尔顿；鲁米诺为177道尔顿)只发挥细胞外效应。
The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only exerts an extracellular effect.