A proteomic investigation into a human gastric cancer cell line BGC823 treated with diallyl trisulfide
A proteomic investigation into a human gastric cancer cell line BGC823 treated with diallyl trisulfide
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DOI：
10.1093/carcin/bgi306
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发表时间：
2006-06-01
期刊：
影响因子：
4.7
通讯作者：
Lu, Youyong
中科院分区：
文献类型：
--
作者：
Li, Na;Guo, Ruifang;Lu, Youyong
大蒜在世界范围内通常被用作治疗各种疾病的治疗剂。尽管人们为了解大蒜及其衍生物的药理机制付出了巨大的努力，但仍有许多谜团有待揭开。本文采用蛋白质组学方法，系统研究了大蒜衍生物的主要成分二烯丙基三硫（DATS）对胃癌细胞BGC 823蛋白质表达的影响。在BGC 823中共检测到41种独特的蛋白质，其表达水平与DATS给药相对应地发生显著变化。其中，谷胱甘肽S-转移酶-π（GST-pi）、电压依赖性阴离子通道-1（VDAC-1）、膜联蛋白1、半乳糖凝集素和S100 A11等5种典型蛋白质，经Western印迹分析，结果与蛋白质组学证据一致。此外，实时定量RT-PCR实验提供了mRNA表达的动态数据，表明DATS处理后短时间内Annexin I和GST-pi表达的反应。有趣的是，BGC 823中类似于50%的DAT敏感蛋白（19/41）与凋亡途径密切相关。因此，这些蛋白质组学结果提供了额外的支持，大蒜是一个强大的诱导肿瘤细胞凋亡的假设。
Garlic is generally used as a therapeutic reagent against various diseases worldwide. Although a great effort is made to understand the pharmaceutical mechanisms of garlic and its derivatives, there are many mysteries to be uncovered. Using proteomic means, herein we have systematically studied the responses of protein expression in BGC823 cells, a gastric cancer cell line, induced by diallyl trisulfide (DATS), a major component of garlic derivatives. A total of 41 unique proteins in BGC823 were detected with significant changes in their expression levels corresponding with DATS administration. Of these proteins, five typical ones, glutathione S-transferase-pi (GST-pi), voltage-dependent anion channel-1 (VDAC-1), Annexin 1, Galectin and S100A11, were further examined by Western blotting, resulting in coincident data with the proteomic evidence. Moreover quantitative real-time RT-PCR experiments offered dynamic data of mRNA expression, indicating the responses of Annexin I and GST-pi expression within a short period after DATS treatment. Interestingly, similar to 50% of DATS-sensitive proteins (19/41) in BGC823 are tightly associated with apoptotic pathways. These proteomic results presented, therefore, provide additional support to the hypothesis that garlic is a strong inducer of apoptosis in tumor cells.