Transcriptome changes in rice (Oryza sativa L.) in response to high night temperature stress at the early milky stage.
Transcriptome changes in rice (Oryza sativa L.) in response to high night temperature stress at the early milky stage.
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水稻（Oryza sativa L.）转录组变化响应乳白期早期夜间高温胁迫
DOI：
10.1186/s12864-015-1222-0
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发表时间：
2015-01-23
期刊：
影响因子：
4.4
通讯作者：
Huang YJ
中科院分区：
文献类型：
--
作者：
Liao JL;Zhou HW;Peng Q;Zhong PA;Zhang HY;He C;Huang YJ
水稻产量和品质受到高温的不利影响，特别是在夜间;夜间高温比白天高温对谷粒重量的危害更大。不幸的是，全球气温一直在以惊人的速度上升，在过去几十年中，夜间最低温度的上升幅度是白天最高温度的三倍。我们利用Illumina测序方法分析了水稻籽粒中耐热和耐热敏感品系在乳熟期早期对夜间高温的反应。对测序数据的分析结果表明，35个转录本在耐热和不耐热水稻中表现出不同的表达，RT-qPCR分析证实了所选转录本的表达模式。对差异表达基因的功能分析表明，21个基因具有功能注释，其功能主要涉及氧化还原过程（6个基因）、代谢过程（7个基因）、转运过程（4个基因）、转录调控过程（2个基因）、防御反应过程（1个基因）和光合过程（1个基因）。在对差异表达基因进行功能注释的基础上，进一步分析了水稻乳熟期前期高夜温胁迫下这些差异表达转录本的调控机制。这一分析表明，夜间高温胁迫破坏了线粒体中的电子传递，导致线粒体和细胞基质中氢离子浓度的变化，并影响植物细胞中TCA及其次生代谢相关酶的活性。利用Illumina测序技术，首次揭示了水稻乳熟前期耐高温和耐热敏感品系在夜间高温胁迫下转录组的差异。这些候选转录本可能为水稻耐热性状的改良提供有用的遗传资源。本文提出的模型是基于两个水稻品系之间的表达和转录差异。此外，该模型可能支持未来的研究植物对夜间高温反应的分子机制。本文的在线版本（doi：10.1186/s12864-015-1222-0）包含补充材料，可供授权用户使用。
Rice yield and quality are adversely affected by high temperatures, especially at night; high nighttime temperatures are more harmful to grain weight than high daytime temperatures. Unfortunately, global temperatures are consistently increasing at an alarming rate and the minimum nighttime temperature has increased three times as much as the corresponding maximum daytime temperature over the past few decades. We analyzed the transcriptome profiles for rice grain from heat-tolerant and -sensitive lines in response to high night temperatures at the early milky stage using the Illumina Sequencing method. The analysis results for the sequencing data indicated that 35 transcripts showed different expressions between heat-tolerant and -sensitive rice, and RT-qPCR analyses confirmed the expression patterns of selected transcripts. Functional analysis of the differentially expressed transcripts indicated that 21 genes have functional annotation and their functions are mainly involved in oxidation-reduction (6 genes), metabolic (7 genes), transport (4 genes), transcript regulation (2 genes), defense response (1 gene) and photosynthetic (1 gene) processes. Based on the functional annotation of the differentially expressed genes, the possible process that regulates these differentially expressed transcripts in rice grain responding to high night temperature stress at the early milky stage was further analyzed. This analysis indicated that high night temperature stress disrupts electron transport in the mitochondria, which leads to changes in the concentration of hydrogen ions in the mitochondrial and cellular matrix and influences the activity of enzymes involved in TCA and its secondary metabolism in plant cells. Using Illumina sequencing technology, the differences between the transcriptomes of heat-tolerant and -sensitive rice lines in response to high night temperature stress at the early milky stage was described here for the first time. The candidate transcripts may provide genetic resources that may be useful in the improvement of heat-tolerant characters of rice. The model proposed here is based on differences in expression and transcription between two rice lines. In addition, the model may support future studies on the molecular mechanisms underlying plant responses to high night temperatures. The online version of this article (doi:10.1186/s12864-015-1222-0) contains supplementary material, which is available to authorized users.
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影响因子：
4.8
作者：
Fu Guan-fu;Song Jian;Tao Long-xing
通讯作者：
Tao Long-xing
影响因子：
3.9
作者：
Iyanagi, Takashi;Xia, Chuanwu;Kim, Jung-Ja P.
通讯作者：
Kim, Jung-Ja P.
影响因子：
4.2
作者：
Cheng, FM;Zhong, LJ;Zhang, GP
通讯作者：
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DOI：
10.1007/978-1-60761-944-4_10
发表时间：
2011-01-01
期刊：
PCR PROTOCOLS, THIRD EDITION
影响因子：
--
作者：
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通讯作者：
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DOI：
10.1073/pnas.0909198107
发表时间：
2010-02-23
影响因子：
11.1
作者：
Li, Lei;Ye, Huaxun;Yin, Yanhai
通讯作者：
Yin, Yanhai