THE ZNF35 HUMAN ZINC FINGER GENE ENCODES A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN
THE ZNF35 HUMAN ZINC FINGER GENE ENCODES A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN
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DOI：
10.1016/0014-5793(93)80115-b
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发表时间：
1993-04-26
期刊：
影响因子：
3.5
通讯作者：
LANIA, L
中科院分区：
文献类型：
--
作者：
PENGUE, G;CANNADABARTOLI, P;LANIA, L
我们开发了一种快速的方法来确定推定的DNA结合蛋白的DNA结合位点。该方法已成功地用于确定人ZNF 35锌指基因的特异性共有位点。ZNF 35编码一个58-kDA的多肽，其氨基端含有11个连续的指状结构域，羧基端含有一个酸性结构域。这些特征表明ZNF 35是一个位点特异性的DNA结合蛋白，参与基因表达的调控。我们从大肠杆菌中表达了ZNF 35蛋白。大肠杆菌，并采用了西南聚合酶链反应方法，使用随机寡核苷酸，以确定其高亲和力结合位点。ZNF 35蛋白结合位点的核心序列为5 '-C/GC/GAAG/TA-3'。
We developed a rapid method to determine DNA-binding sites for putative DNA-binding proteins. This procedure has been successfully used to define a specific consensus site for the human ZNF35 zinc finger gene. ZNF35 encodes a 58-kDA polypeptide containing 11 consecutive finger motifs located at the amino terminus, and an acidic domain located at the carboxy terminus. These features suggest that ZNF35 is a site-specific DNA-binding protein involved in the regulation of gene expression. We have expressed the ZNF35 protein from E. coli and have employed a Southwestern-polymerase chain reaction method using random oligonucleotides to identify its high-affinity binding site. The core sequence for the ZNF35 protein-binding site is 5'-C/GC/GAAG/TA-3'.