DEPENDENCE OF HEPATOCYTE-SPECIFIC GENE-EXPRESSION ON CELL-CELL INTERACTIONS IN PRIMARY CULTURE
DEPENDENCE OF HEPATOCYTE-SPECIFIC GENE-EXPRESSION ON CELL-CELL INTERACTIONS IN PRIMARY CULTURE
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DOI：
10.1002/j.1460-2075.1985.tb03960.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
GUGUENGUILLOUZO, C
中科院分区：
文献类型：
--
作者：
FRASLIN, JM;KNEIP, B;GUGUENGUILLOUZO, C
在与非实质肝上皮细胞共培养时，大鼠肝细胞的白蛋白和总蛋白合成显著增加，与白蛋白分泌早期下降的纯细胞相比。通过杂交确定的不同mRNA序列的相对数量的分析表明，蛋白质合成的增加主要是由于相应的mRNAs水平的增加。此外，当两种细胞在肝细胞接种后几天建立细胞-细胞接触时，同样地观察到白蛋白分泌的刺激，相应的mRNA在培养的第10-14天显著增加。用分离的细胞核研究RNA合成的转录分析表明，肝脏特异性基因的转录显著增加，并保持至少2周。这些结果首次证明了成人肝细胞在原代培养中高水平的各种特定的mRNAs的长期稳定性和可逆性。他们认为，在肝细胞和肝上皮细胞之间建立细胞-细胞接触对于维持肝脏特异基因的高转录速率是必不可少的。
In co-culture with non-parenchymal liver epithelial cells, rat hepatocytes show a marked increase in albumin and total protein synthesis when compared with cells maintained as pure populations in which an early decline in albumin secretion takes place. Analysis of the relative amounts of different mRNA sequences, determined by hybridization, indicated that the increase in protein synthesis resulted essentially from an increased level of the corresponding mRNAs. In addition, when cell-cell contacts were established between the two cell types several days after the seeding of hepatocytes, the stimulation of albumin secretion was similarly observed with a significant increase of the corresponding mRNA on days 10-14 of culture. Transcriptional assays, in which isolated nuclei were used for the study of RNA synthesis, showed that liver specific gene transcription was significantly increased and maintained for at least 2 weeks. These results demonstrate for the first time long-term stabilization and reversibility of various specific mRNAs at high levels by adult hepatocytes in primary culture. They suggest that establishment of cell-cell contacts between hepatocytes and liver epithelial cells are essential for the maintenance of a high rate of transcription of the liver-specific genes.