Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones
Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones
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DOI：
10.1016/s0015-0282(01)01986-0
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发表时间：
2001-10-01
影响因子：
6.7
通讯作者：
Azziz, R
中科院分区：
文献类型：
--
作者：
Reyna, R;Traynor, KD;Azziz, R
目的：确定反复冻融和储存温度对人血清中通常测量的生殖激素和性激素结合球蛋白（SHBG）测定结果的影响。设计：前瞻性实验室研究。设置：学术医学中心。患者：4名男性和3名妊娠晚期女性。干预：将来自男性和妊娠女性的混合血清冷冻在-20 ℃或-70 ℃下。然后使等分试样经受重复冻融循环，从1至10次，并且在最终冻融循环后进行测定。（FSH、LH、PRL、雄烯二酮（A）、17 α-羟孕酮、P、胰岛素和SHBG，作为冻融循环次数和储存温度的函数。只有男性血清中的SHBG（-20 ℃）和妊娠血清中的P（-70 ℃）在重复冻融循环中显示出统计学显著性降低（每个循环分别为3.3%和1.1%）。所有其他分析物没有表现出显着的变化作为冻/融循环或存储temperature. Conclusions（S）的函数：有没有一致的或可预测的改变，在SHBG的结果，或糖蛋白和类固醇激素的评价，作为反复冻/融或人血清的存储温度的函数。（Fertil Steril（R）2001;76：823-5. (C)2001年由美国生殖医学学会出版。
Objective: To determine the effect of repeated freezing and thawing, and storage temperature, on the assay results of commonly measured reproductive hormones, and sex hormone-binding globulin (SHBG) in human serum.Design: Prospective laboratory study.Setting: Academic medical center.Patient(s): Four men and three pregnant women in the third trimester.Intervention(s): Pooled serum from men and pregnant females were frozen at either -20 degreesC or -70 degreesC. Aliquots were then subjected to repeat freeze/thaw cycles, from 1 to 10 times, and assays were performed after the final freeze/thaw cycle.Main Outcome Measure(s): Assay results for eight hormones (FSH, LH, PRL, androstenedione (A), 17 alpha -hydroxyprogesterone, P, insulin, and SHBG, as a function of the number of freeze/thaw cycles and storage temperature.Result(s): Only SHBG in male serum at -20 degreesC and P in pregnant serum at -70 degreesC showed statistically significant decreases in assay results with repeated freeze/thaw cycles (3.3% and 1.1% per cycle, respectively). All other analytes did not show significant changes as a function of freeze/thaw cycles or storage temperature.Conclusion(s): There is no consistent or predictable alteration in the results of SHBG, or the glycoprotein and steroid hormones evaluated, as a function of repeated freeze/thaw or the storage temperature of human serum. (Fertil Steril(R) 2001;76:823-5. (C) 2001 by American Society for Reproductive Medicine.).